PMID- 22464918
OWN - NLM
STAT- MEDLINE
DCOM- 20120926
LR  - 20191210
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 35
IP  - 1
DP  - 2012 May 15
TI  - Layer-by-layer assembly of chemical reduced graphene and carbon nanotubes for 
      sensitive electrochemical immunoassay.
PG  - 63-68
LID - S0956-5663(12)00067-X [pii]
LID - 10.1016/j.bios.2012.02.007 [doi]
AB  - In this work, uniform and stable multi-walled carbon nanotubes (MWCT) and 
      chemically reduced graphene (GR) composite electrode interface was fabricated by 
      using layer-by-layer assembly method. The performances of these GR-MWCT assembled 
      electrode interfaces were studied by electrochemical impedance spectroscopy (EIS) 
      and cyclic voltammetry (CV). It was demonstrated that the assembled composite 
      film significantly improved the interfacial electron transfer rate compared with 
      that of GR or MWCT modified electrode. Based on the GR-MWCT assembled interface, 
      a sandwich-type electrochemical immunosensor was constructed using human IgG as a 
      model target. In this assay, human IgG was fixed as the target antigen, the 
      HRP-conjugated IgG as the probing antibody and hydroquinone as the electron 
      mediator. The detection limit of the immunosensor was 0.2 ng mL(-1) 
      (signal-to-noise ratio of 3). A good linear relationship between the current 
      signals and the concentrations of Human IgG was achieved from 1 ng mL(-1) to 500 
      ng mL(-1). Moreover, this electrochemical immunosensor exhibited excellent 
      selectivity, stability and reproducibility, and can be used to accurately detect 
      IgG concentration in human serum samples. The results suggest that the 
      electrochemical immunosensor based on GR-MWCT assembled composite will be 
      promising in the point-of-care diagnostics application of clinical screening of 
      multiple diseases.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Liu, Yan
AU  - Liu Y
AD  - Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and 
      Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical 
      Biology, Tsinghua University, Beijing 100084, China; Department of Chemistry and 
      Chemical Engineering, Yangtze Normal University, Fuling, Chongqing 408100, China.
FAU - Liu, Yang
AU  - Liu Y
AD  - Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and 
      Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical 
      Biology, Tsinghua University, Beijing 100084, China. Electronic address: 
      liu-yang@mail.tsinghua.edu.cn.
FAU - Feng, Hongbin
AU  - Feng H
AD  - Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and 
      Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical 
      Biology, Tsinghua University, Beijing 100084, China.
FAU - Wu, Yongmin
AU  - Wu Y
AD  - Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and 
      Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical 
      Biology, Tsinghua University, Beijing 100084, China.
FAU - Joshi, Lokesh
AU  - Joshi L
AD  - Alimentary Glycoscience Research Cluster, National Centre for Biomedical 
      Engineering Science, National University of Ireland, Galway, Ireland.
FAU - Zeng, Xiangqun
AU  - Zeng X
AD  - Chemistry Department, Oakland University, Rochester, MI 48309, USA.
FAU - Li, Jinghong
AU  - Li J
AD  - Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and 
      Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical 
      Biology, Tsinghua University, Beijing 100084, China. Electronic address: 
      jhli@mail.tsinghua.edu.cn.
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120307
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Immunoglobulin G)
RN  - 0 (Nanotubes, Carbon)
RN  - 7782-42-5 (Graphite)
SB  - IM
MH  - Biosensing Techniques/instrumentation/*methods
MH  - Dielectric Spectroscopy
MH  - Electrochemical Techniques
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Equipment Design
MH  - *Graphite
MH  - Humans
MH  - Immunoassay/instrumentation/*methods
MH  - Immunoglobulin G/analysis/blood
MH  - *Nanotubes, Carbon
EDAT- 2012/04/03 06:00
MHDA- 2012/09/27 06:00
CRDT- 2012/04/03 06:00
PHST- 2011/11/28 00:00 [received]
PHST- 2012/02/02 00:00 [revised]
PHST- 2012/02/06 00:00 [accepted]
PHST- 2012/04/03 06:00 [entrez]
PHST- 2012/04/03 06:00 [pubmed]
PHST- 2012/09/27 06:00 [medline]
AID - S0956-5663(12)00067-X [pii]
AID - 10.1016/j.bios.2012.02.007 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2012 May 15;35(1):63-68. doi: 10.1016/j.bios.2012.02.007. 
      Epub 2012 Mar 7.