PMID- 22465611
OWN - NLM
STAT- MEDLINE
DCOM- 20120629
LR  - 20211021
IS  - 1090-2449 (Electronic)
IS  - 0014-4894 (Print)
IS  - 0014-4894 (Linking)
VI  - 131
IP  - 1
DP  - 2012 May
TI  - Characterization of a Trypanosoma brucei Alkb homolog capable of repairing 
      alkylated DNA.
PG  - 92-100
LID - 10.1016/j.exppara.2012.03.011 [doi]
AB  - Trypanosoma brucei encodes a protein (denoted TbABH) that is homologous to AlkB 
      of Escherichia coli and AlkB homolog (ABH) proteins in other organisms, raising 
      the possibility that trypanosomes catalyze oxidative repair of alkylation-damaged 
      DNA. TbABH was cloned and expressed in E. coli, and the recombinant protein was 
      purified and characterized. Incubation of anaerobic TbABH with Fe(II) and 
      alpha-ketoglutarate (alphaKG) produces a characteristic metal-to-ligand charge-transfer 
      chromophore, confirming its membership in the Fe(II)/alphaKG dioxygenase superfamily. 
      The protein binds to DNA, with a clear preference for alkylated oligonucleotides 
      according to results derived by electrophoretic mobility shift assays. Finally, 
      the protozoan gene was shown to partially complement E. coli alkB cells when 
      stressed with methylmethanesulfonate; thus confirming assignment of TbABH as a 
      functional AlkB protein in T. brucei.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Simmons, Jana M
AU  - Simmons JM
AD  - Department of Biochemistry & Molecular Biology, Michigan State University, East 
      Lansing, MI, United States.
FAU - Koslowsky, Donna J
AU  - Koslowsky DJ
FAU - Hausinger, Robert P
AU  - Hausinger RP
LA  - eng
GR  - R01 AI045835-05/AI/NIAID NIH HHS/United States
GR  - R01 GM063584-10/GM/NIGMS NIH HHS/United States
GR  - AI45835/AI/NIAID NIH HHS/United States
GR  - GM063584/GM/NIGMS NIH HHS/United States
GR  - R01 AI045835/AI/NIAID NIH HHS/United States
GR  - R01 GM063584/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20120320
PL  - United States
TA  - Exp Parasitol
JT  - Experimental parasitology
JID - 0370713
RN  - 0 (DNA, Protozoan)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Protozoan Proteins)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Alkylation
MH  - Amino Acid Sequence
MH  - Chromatography, Gel
MH  - Cloning, Molecular
MH  - DNA Repair/*physiology
MH  - DNA, Protozoan/chemistry/*genetics
MH  - DNA-Binding Proteins/chemistry/genetics/physiology
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Electrophoretic Mobility Shift Assay
MH  - Escherichia coli
MH  - Gene Expression
MH  - Genetic Complementation Test
MH  - Humans
MH  - Molecular Sequence Data
MH  - Oxidation-Reduction
MH  - Protozoan Proteins/chemistry/genetics/*physiology
MH  - Recombinant Proteins/chemistry/genetics/isolation & purification
MH  - Sequence Alignment
MH  - Spectrophotometry, Ultraviolet
MH  - Trypanosoma brucei brucei/chemistry/*genetics
PMC - PMC3340446
MID - NIHMS365647
EDAT- 2012/04/03 06:00
MHDA- 2012/06/30 06:00
PMCR- 2013/05/01
CRDT- 2012/04/03 06:00
PHST- 2012/01/16 00:00 [received]
PHST- 2012/02/29 00:00 [revised]
PHST- 2012/03/12 00:00 [accepted]
PHST- 2012/04/03 06:00 [entrez]
PHST- 2012/04/03 06:00 [pubmed]
PHST- 2012/06/30 06:00 [medline]
PHST- 2013/05/01 00:00 [pmc-release]
AID - S0014-4894(12)00105-1 [pii]
AID - 10.1016/j.exppara.2012.03.011 [doi]
PST - ppublish
SO  - Exp Parasitol. 2012 May;131(1):92-100. doi: 10.1016/j.exppara.2012.03.011. Epub 
      2012 Mar 20.