PMID- 22466340
OWN - NLM
STAT- MEDLINE
DCOM- 20120815
LR  - 20240318
IS  - 1945-7197 (Electronic)
IS  - 0021-972X (Print)
IS  - 0021-972X (Linking)
VI  - 97
IP  - 6
DP  - 2012 Jun
TI  - Transforming growth factor beta1 (TGFbeta1) and progesterone regulate matrix 
      metalloproteinases (MMP) in human endometrial stromal cells.
PG  - E888-97
LID - 10.1210/jc.2011-3073 [doi]
AB  - CONTEXT: Menstruation is preceded by progesterone withdrawal and endometrial 
      matrix remodeling predominantly through induction of matrix metalloproteinases 
      (MMP) and recruitment of invading neutrophils. DESIGN: Using endometrial tissues 
      from women during various phases of the menstrual cycle, we found that MMP2, 
      MMP9, and MMP11 were up-regulated in the late secretory phase/premenstrual phase. 
      Because TGFbeta-responsive genes were also up-regulated in endometrium during this 
      time, we tested the hypothesis that TGFbeta1 and progesterone regulate expression of 
      MMP in human endometrial stromal cells (HESC). RESULTS: Treatment of HESC with 
      TGFbeta1 resulted in marked increases in MMP2 and MMP11 mRNA and pro- and active 
      MMP2 activity. Progesterone inhibited TGFbeta1-induced stimulation of MMP2 and MMP11 
      through its nuclear hormone receptors. Interestingly, TGFbeta1 also decreased 
      progesterone receptor (PR)-A and PR-B in HESC with a more pronounced effect on 
      PR-A. CONCLUSIONS: These data support the hypothesis that TGFbeta1 has endogenous 
      anti-progestational effects in HESC and that the opposing effects of progesterone 
      and TGFbeta1 are important in regulation of matrix integrity in human endometrium.
FAU - Itoh, Hiroko
AU  - Itoh H
AD  - Department of Obstetrics and Gynecology, University of Texas Southwestern Medical 
      Center, 5323 Harry Hines Boulevard, Dallas, Texas 75235-9032, USA.
FAU - Kishore, Annavarapu Hari
AU  - Kishore AH
FAU - Lindqvist, Annika
AU  - Lindqvist A
FAU - Rogers, David E
AU  - Rogers DE
FAU - Word, R Ann
AU  - Word RA
LA  - eng
GR  - P01 HD011149/HD/NICHD NIH HHS/United States
GR  - HD11149/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20120330
PL  - United States
TA  - J Clin Endocrinol Metab
JT  - The Journal of clinical endocrinology and metabolism
JID - 0375362
RN  - 0 (Transforming Growth Factor beta1)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - EC 3.4.24.- (MMP11 protein, human)
RN  - EC 3.4.24.- (Matrix Metalloproteinase 11)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
RN  - EC 3.4.24.24 (MMP2 protein, human)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Adult
MH  - Endometrium/cytology/*metabolism
MH  - Female
MH  - Gene Expression Regulation, Enzymologic/drug effects/physiology
MH  - Humans
MH  - In Vitro Techniques
MH  - Matrix Metalloproteinase 11/genetics/metabolism
MH  - Matrix Metalloproteinase 2/genetics/metabolism
MH  - Matrix Metalloproteinase 9/genetics/metabolism
MH  - Matrix Metalloproteinases/genetics/*metabolism
MH  - Menstrual Cycle/*metabolism/physiology
MH  - Middle Aged
MH  - Progesterone/*metabolism/pharmacology
MH  - Stromal Cells/cytology/drug effects/*metabolism
MH  - Transforming Growth Factor beta1/*metabolism/pharmacology
PMC - PMC3387423
EDAT- 2012/04/03 06:00
MHDA- 2012/08/16 06:00
PMCR- 2013/06/01
CRDT- 2012/04/03 06:00
PHST- 2012/04/03 06:00 [entrez]
PHST- 2012/04/03 06:00 [pubmed]
PHST- 2012/08/16 06:00 [medline]
PHST- 2013/06/01 00:00 [pmc-release]
AID - jc.2011-3073 [pii]
AID - 11-3073 [pii]
AID - 10.1210/jc.2011-3073 [doi]
PST - ppublish
SO  - J Clin Endocrinol Metab. 2012 Jun;97(6):E888-97. doi: 10.1210/jc.2011-3073. Epub 
      2012 Mar 30.