PMID- 22466886
OWN - NLM
STAT- MEDLINE
DCOM- 20121022
LR  - 20191027
IS  - 1880-4926 (Electronic)
IS  - 1343-4934 (Linking)
VI  - 54
IP  - 1
DP  - 2012 Mar
TI  - Role of CTGF/CCN2 in reparative dentinogenesis in human dental pulp.
PG  - 47-54
AB  - Connective tissue growth factor/CCN family 2 (CTGF/CCN2) has been considered to 
      participate in tooth development. To date, the expression and role of CTGF/CCN2 
      in reparative dentinogenesis have been unclear. Our previous study revealed that 
      matrix metalloproteinase-3 (MMP-3) stimulates cell migration via CTGF/CCN2 
      expression and secretion in human dental pulp cells, and that this is dependent 
      on dynamin-related endocytosis and independent of protease activity. The 
      objective of the present study was to determine the expression of CTGF/CCN2 in 
      reparative dentin in human carious teeth and to examine the effect of CTGF/CCN2 
      on mineralization in cultured human dental pulp cells. Minimal expression of 
      CTGF/CCN2 was evident in odontoblasts subjacent to the dentin-pulp junction in 
      healthy teeth, whereas strong expression was detected in odontoblast-like cells 
      lining the reparative dentin subjacent to dental caries. In human dental pulp 
      cells, CTGF/CCN2 promoted mineralization but failed to induce proliferation, 
      suggesting that this molecule has the ability to induce the differentiation of 
      human dental pulp cells. Taken together, the data suggest that CTGF/CCN2 is 
      likely involved in reparative dentinogenesis through formation of hard tissue in 
      human carious teeth.
FAU - Muromachi, Koichiro
AU  - Muromachi K
AD  - Department of Endodontics, Nihon University School of Dentistry at Matsudo, 
      Japan. mako08014@g.nihon-u.ac.jp
FAU - Kamio, Naoto
AU  - Kamio N
FAU - Matsumoto, Takashi
AU  - Matsumoto T
FAU - Matsushima, Kiyoshi
AU  - Matsushima K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - J Oral Sci
JT  - Journal of oral science
JID - 9808942
RN  - 0 (CCN2 protein, human)
RN  - 0 (Recombinant Proteins)
RN  - 139568-91-5 (Connective Tissue Growth Factor)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Analysis of Variance
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Connective Tissue Growth Factor/chemistry/*physiology
MH  - Dental Caries/*metabolism
MH  - Dental Pulp/cytology/*metabolism
MH  - Dentin, Secondary/growth & development/*metabolism
MH  - Humans
MH  - Matrix Metalloproteinase 3/physiology
MH  - Odontoblasts/metabolism
MH  - Protein Structure, Tertiary
MH  - Recombinant Proteins/pharmacology
MH  - Tooth Calcification/physiology
MH  - Young Adult
EDAT- 2012/04/03 06:00
MHDA- 2012/10/23 06:00
CRDT- 2012/04/03 06:00
PHST- 2012/04/03 06:00 [entrez]
PHST- 2012/04/03 06:00 [pubmed]
PHST- 2012/10/23 06:00 [medline]
AID - JST.JSTAGE/josnusd/54.47 [pii]
AID - 10.2334/josnusd.54.47 [doi]
PST - ppublish
SO  - J Oral Sci. 2012 Mar;54(1):47-54. doi: 10.2334/josnusd.54.47.