PMID- 22467034
OWN - NLM
STAT- MEDLINE
DCOM- 20120807
LR  - 20210816
IS  - 1880-3989 (Electronic)
IS  - 0388-1350 (Linking)
VI  - 37
IP  - 2
DP  - 2012
TI  - Evaluation of in vitro screening system for estrogenicity: comparison of stably 
      transfected human estrogen receptor-alpha transcriptional activation (OECD TG455) 
      assay and estrogen receptor (ER) binding assay.
PG  - 431-7
AB  - The estrogenic activity of industrial chemicals, di(2-ethylhexyl) phthalate 
      (DEHP), di(n-butyl) phthalate (DBP), benzylbutyl phthalate (BBP), diethyl 
      phthalate (DEP), tetrabromobisphenol A (TBBPA), bisphenol A (BPA), and 
      nonylphenol (NP), was compared using OECD test guideline 455(TG455), stably 
      transfected transcriptional activation (STTA) and estrogen receptor (ER) binding 
      assays. The estrogenic activity of BBP, BPA and NP were approximately 
      180,000-fold (PC(50), 4.32 x 10(-6 )M), 5,000-fold (PC(50), 1.26 x 10(-7) M) and 
      120,000-fold (PC(50), 2.92 x 10(-6 )M) less than 17beta-estradiol (PC(50), 2.43 x 
      10(-11)M), whereas DEHP, DBP and DEP did not show any estrogenicity activity in 
      the STTA assay. Moreover, binding affinities to human ERalpha of BBP, BPA, and NP 
      were approximately 200,000-fold (IC(50), 4.91 x 10(-4) M), 8000-fold (IC(50), 
      1.92 x 10(-5) M) and 1400-fold (IC(50), 3.34 x 10(-6) M) less than 17beta-estradiol 
      (IC(50), 2.45 x 10(-9) M) in competitive human ERalpha binding assay. The relative 
      potencies of STTA assay were very similar to ER binding, E-screen, and Yeast 
      screening assays. Therefore, our results suggested that OECD test guideline TG455 
      may be useful as a screening test for potential endocrine disruptors.
FAU - Lee, Hae Kyung
AU  - Lee HK
FAU - Kim, Tae Sung
AU  - Kim TS
FAU - Kim, Chang Yeong
AU  - Kim CY
FAU - Kang, Il Hyun
AU  - Kang IH
FAU - Kim, Mi Gyeong
AU  - Kim MG
FAU - Jung, Ki Kyung
AU  - Jung KK
FAU - Kim, Hyung Sik
AU  - Kim HS
FAU - Han, Soon Young
AU  - Han SY
FAU - Yoon, Hae Jung
AU  - Yoon HJ
FAU - Rhee, Gyu Seek
AU  - Rhee GS
LA  - eng
PT  - Letter
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - J Toxicol Sci
JT  - The Journal of toxicological sciences
JID - 7805798
RN  - 0 (Benzhydryl Compounds)
RN  - 0 (ESR1 protein, human)
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Estrogens)
RN  - 0 (Phenols)
RN  - 0 (Phthalic Acids)
RN  - 0 (Polybrominated Biphenyls)
RN  - 0 (Recombinant Proteins)
RN  - 79F6A2ILP5 (nonylphenol)
RN  - FQI02RFC3A (tetrabromobisphenol A)
RN  - MLT3645I99 (bisphenol A)
SB  - IM
MH  - Benzhydryl Compounds
MH  - Binding, Competitive
MH  - Biological Assay
MH  - Estrogen Receptor alpha/genetics/*metabolism
MH  - Estrogens/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Phenols/*metabolism
MH  - Phthalic Acids/*metabolism
MH  - Polybrominated Biphenyls/*toxicity
MH  - Recombinant Proteins/metabolism
MH  - Transcriptional Activation
EDAT- 2012/04/03 06:00
MHDA- 2012/08/08 06:00
CRDT- 2012/04/03 06:00
PHST- 2012/04/03 06:00 [entrez]
PHST- 2012/04/03 06:00 [pubmed]
PHST- 2012/08/08 06:00 [medline]
AID - JST.JSTAGE/jts/37.431 [pii]
AID - 10.2131/jts.37.431 [doi]
PST - ppublish
SO  - J Toxicol Sci. 2012;37(2):431-7. doi: 10.2131/jts.37.431.