PMID- 22467162
OWN - NLM
STAT- MEDLINE
DCOM- 20120913
LR  - 20220408
IS  - 1097-0223 (Electronic)
IS  - 0197-3851 (Linking)
VI  - 32
IP  - 4
DP  - 2012 Apr
TI  - Same-day prenatal diagnosis of common chromosomal aneuploidies using 
      microfluidics-fluorescence in situ hybridization.
PG  - 321-8
LID - 10.1002/pd.2946 [doi]
AB  - Rapid molecular prenatal diagnostic methods, such as fluorescence in situ 
      hybridization (FISH), quantitative fluorescence-PCR, and multiplex 
      ligation-dependent probe amplification, can detect common fetal aneuploidies 
      within 24 to 48 h. However, specific diagnosis or aneuploidy exclusion should be 
      ideally available within the same day as fetal sampling to alleviate parental 
      anxiety. Microfluidic technologies integrate different steps into a microchip, 
      saving time and costs. We have developed a cost-effective, same-day prenatal 
      diagnostic FISH assay using microfluidics. Amniotic fluids (1-4 mL from 40 
      pregnant women at 15-22 weeks of gestation) were fixed with Carnoy's before 
      loading into the microchannels of a microfluidic FISH-integrated nanostructured 
      device. The glass slides were coated with nanostructured titanium dioxide to 
      facilitate cell adhesion. Pretreatment and hybridization were performed within 
      the microchannels. Fifty nuclei were counted by two independent analysts, and all 
      results were validated with their respective karyotypes. Of the 40 samples, we 
      found three cases of fetal aneuploidies (trisomies 13, 18, and 21), whereas the 
      remaining 37 cases were normal. Results were concordant with their karyotypes and 
      ready to be released within 3 h of sample receipt. Microfluidic FISH, using 
      20-fold less than the recommended amount of probe, is a cost-effective method to 
      diagnose common fetal aneuploidies within the same day of fetal sampling.
CI  - Copyright (c) 2011 John Wiley & Sons, Ltd.
FAU - Ho, Sherry S Y
AU  - Ho SS
AD  - Department of Laboratory Medicine, National University Hospital, Singapore, 
      Singapore.
FAU - Chua, Cuiwen
AU  - Chua C
FAU - Gole, Leena
AU  - Gole L
FAU - Biswas, Arijit
AU  - Biswas A
FAU - Koay, Evelyn
AU  - Koay E
FAU - Choolani, Mahesh
AU  - Choolani M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
SB  - IM
MH  - Adult
MH  - Amniocentesis/economics/*methods
MH  - Amniotic Fluid/*cytology
MH  - *Aneuploidy
MH  - Cell Count
MH  - Chromosome Disorders/*diagnosis/genetics
MH  - Cost-Benefit Analysis
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Maternal Age
MH  - Microfluidics
MH  - Molecular Diagnostic Techniques
MH  - Nuchal Translucency Measurement
MH  - Pregnancy
MH  - Pregnancy Trimester, Second
MH  - Reproducibility of Results
MH  - Time Factors
EDAT- 2012/04/03 06:00
MHDA- 2012/09/14 06:00
CRDT- 2012/04/03 06:00
PHST- 2012/04/03 06:00 [entrez]
PHST- 2012/04/03 06:00 [pubmed]
PHST- 2012/09/14 06:00 [medline]
AID - 10.1002/pd.2946 [doi]
PST - ppublish
SO  - Prenat Diagn. 2012 Apr;32(4):321-8. doi: 10.1002/pd.2946.