PMID- 22467423
OWN - NLM
STAT- MEDLINE
DCOM- 20140710
LR  - 20131010
IS  - 1932-7005 (Electronic)
IS  - 1932-6254 (Linking)
VI  - 7
IP  - 10
DP  - 2013 Oct
TI  - Liver progenitor cell interactions with the extracellular matrix.
PG  - 757-66
LID - 10.1002/term.1470 [doi]
AB  - Liver progenitor cells (LPCs) are a promising source of cells to treat liver 
      disease by cell therapy, due to their capability for self-replication and 
      bipotentiality. In order to establish useful culture systems of LPCs and apply 
      them to future clinical therapies, it is necessary to understand their 
      interactions with their microenvironment and especially with the extracellular 
      matrix (ECM). There is considerable evidence from in vivo studies that matrix 
      proteins affect the activation, expansion, migration and differentiation of LPCs, 
      but the information on the role that specific ECMs play in regulating LPCs in 
      vitro is more limited. Nevertheless, current studies suggest that laminin, 
      collagen type III, collagen type IV and hyaluronic acid help to maintain the 
      undifferentiated phenotype of LPCs and promote their proliferation when cultured 
      in media supplemented with growth factors chosen for LPC expansion, whereas 
      collagen type I and fibronectin are generally associated with a differentiated 
      phenotype under the same conditions. Experimental evidence suggests that alpha6beta1 and 
      alpha5beta1 integrins as well as CD44 on the surface of LPCs, and their related 
      downstream signals, are important mediators of interactions between LPCs and the 
      ECM. The interactions of LPCs with the ECM form the focus of this review and the 
      contribution of ECM molecules to strategies for optimizing in vitro LPC cultures 
      for therapeutic applications is discussed.
CI  - Copyright (c) 2012 John Wiley & Sons, Ltd.
FAU - Zhu, Chunxia
AU  - Zhu C
AD  - State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First 
      Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, People's 
      Republic of China.
FAU - Coombe, Deirdre R
AU  - Coombe DR
FAU - Zheng, Ming H
AU  - Zheng MH
FAU - Yeoh, George C T
AU  - Yeoh GC
FAU - Li, Lanjuan
AU  - Li L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20120330
PL  - England
TA  - J Tissue Eng Regen Med
JT  - Journal of tissue engineering and regenerative medicine
JID - 101308490
SB  - IM
MH  - Animals
MH  - *Cell Communication
MH  - Cell Culture Techniques
MH  - Extracellular Matrix/*metabolism
MH  - Humans
MH  - Liver/*cytology
MH  - Stem Cells/*cytology/physiology
OTO - NOTNLM
OT  - extracellular matrix
OT  - integrins
OT  - interactions
OT  - liver progenitor cells
OT  - matrix receptors
OT  - matrix turnover
EDAT- 2012/04/03 06:00
MHDA- 2014/07/11 06:00
CRDT- 2012/04/03 06:00
PHST- 2011/06/25 00:00 [received]
PHST- 2011/10/26 00:00 [revised]
PHST- 2012/01/05 00:00 [accepted]
PHST- 2012/04/03 06:00 [entrez]
PHST- 2012/04/03 06:00 [pubmed]
PHST- 2014/07/11 06:00 [medline]
AID - 10.1002/term.1470 [doi]
PST - ppublish
SO  - J Tissue Eng Regen Med. 2013 Oct;7(10):757-66. doi: 10.1002/term.1470. Epub 2012 
      Mar 30.