PMID- 22468989
OWN - NLM
STAT- MEDLINE
DCOM- 20130501
LR  - 20181201
IS  - 1574-6941 (Electronic)
IS  - 0168-6496 (Linking)
VI  - 81
IP  - 3
DP  - 2012 Sep
TI  - Ultrastructural and molecular characterization of a bacterial symbiosis in the 
      ecologically important scale insect family Coelostomidiidae.
PG  - 537-46
LID - 10.1111/j.1574-6941.2012.01378.x [doi]
AB  - Scale insects are important ecologically and as agricultural pests. The majority 
      of scale insect taxa feed exclusively on plant phloem sap, which is carbon rich 
      but deficient in essential amino acids. This suggests that, as seen in the 
      related aphids and psyllids, scale insect nutrition might also depend upon 
      bacterial symbionts, yet very little is known about scale insect-bacteria 
      symbioses. We report here the first identification and molecular characterization 
      of symbiotic bacteria associated with the New Zealand giant scale Coelostomidia 
      wairoensis, using fluorescence in situ hybridization (FISH), transmission 
      electron microscopy (TEM) and 16S rRNA gene-based analysis. Dissection and FISH 
      confirmed the location of the bacteria in large, paired, multilobate organs in 
      the abdominal region of the insect. TEM indicated that the dominant pleomorphic 
      bacteria were confined to bacteriocytes in the sheath-enclosed bacteriome. 
      Phylogenetic analysis revealed the presence of three distinct bacterial types, 
      the bacteriome-associated B-symbiont (Bacteroidetes), an Erwinia-related symbiont 
      (Gammaproteobacteria) and Wolbachia sp. (Alphaproteobacteria). This study extends 
      the current knowledge of scale insect symbionts and is the first microbiological 
      investigation of the ecologically important coelostomidiid scales.
CI  - (c) 2012 Federation of European Microbiological Societies. Published by Blackwell 
      Publishing Ltd. All rights reserved.
FAU - Dhami, Manpreet K
AU  - Dhami MK
AD  - Centre for Microbial Innovation, School of Biological Sciences, University of 
      Auckland, Auckland, New Zealand. mdha006@aucklanduni.ac.nz
FAU - Turner, Adrian P
AU  - Turner AP
FAU - Deines, Peter
AU  - Deines P
FAU - Beggs, Jacqueline R
AU  - Beggs JR
FAU - Taylor, Michael W
AU  - Taylor MW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120423
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Bacteroidetes/classification/genetics/*isolation & purification/physiology
MH  - Female
MH  - Gammaproteobacteria/classification/genetics/*isolation & purification/physiology
MH  - Hemiptera/*microbiology/physiology
MH  - In Situ Hybridization, Fluorescence
MH  - New Zealand
MH  - Phylogeny
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Symbiosis
MH  - Wolbachia/classification/genetics/*isolation & purification/physiology
EDAT- 2012/04/04 06:00
MHDA- 2013/05/02 06:00
CRDT- 2012/04/04 06:00
PHST- 2012/01/11 00:00 [received]
PHST- 2012/03/25 00:00 [revised]
PHST- 2012/03/28 00:00 [accepted]
PHST- 2012/04/04 06:00 [entrez]
PHST- 2012/04/04 06:00 [pubmed]
PHST- 2013/05/02 06:00 [medline]
AID - 10.1111/j.1574-6941.2012.01378.x [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2012 Sep;81(3):537-46. doi: 
      10.1111/j.1574-6941.2012.01378.x. Epub 2012 Apr 23.