PMID- 22469337
OWN - NLM
STAT- MEDLINE
DCOM- 20130429
LR  - 20120718
IS  - 1574-695X (Electronic)
IS  - 0928-8244 (Linking)
VI  - 65
IP  - 3
DP  - 2012 Aug
TI  - Skewed T-cell receptor beta chain variable gene (TCRBV) usage among different 
      clinical types of patients with chronic HBV infection.
PG  - 448-55
LID - 10.1111/j.1574-695X.2012.00969.x [doi]
AB  - This study aimed to determine the degree of clonal expansion of T cells in 
      peripheral blood mononuclear cells (PBMCs) isolated from patients suffering from 
      different clinical types of hepatitis B (HB) infection and to analyse the 
      clinical relevance of the skewed T-cell receptor beta variable (TCRBV). Sera and 
      PBMCs were collected from 90 HB patients. Gene melting spectral pattern (GMSP) 
      analysis was used to determine the distribution and expansion of populations 
      expressing specific TCRBV complementary determined region 3 (CDR3) genes. TCRBV 
      genes associated with monoclonal expansion were sequenced. TCRBV families from 
      the majority of patients (80/90) displayed skewed T-cell expansion. Furthermore, 
      TCRBV11, BV12 and BV13.1 were more frequent than other TCRBV genes; the sequence 
      of TCRBV11 CDR3 was expressed as 'VYNEQ' in all patients and was accompanied by 
      the BJ2.1 fragment. In patients with chronic HB, the frequency of skewed TCRBV 
      was inversely correlated with hepatitis B virus (HBV) DNA levels. The 
      persistently skewed TCRBV gene families in HB patients may be associated with the 
      development and maintenance of hepatitis. GMSP analysis of TCRBV gene families 
      may be helpful in estimating disease status, and BV11 may be associated with HBV 
      replication in patients with chronic HBV infection.
CI  - (c) 2012 Federation of European Microbiological Societies. Published by Blackwell 
      Publishing Ltd. All rights reserved.
FAU - Yang, Jiezuan
AU  - Yang J
AD  - State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First 
      Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
FAU - Chen, Jiajia
AU  - Chen J
FAU - Mao, Hejun
AU  - Mao H
FAU - Yi, Ping
AU  - Yi P
FAU - Yan, Dong
AU  - Yan D
FAU - He, Jianqin
AU  - He J
FAU - Li, Lanjuan
AU  - Li L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120521
PL  - England
TA  - FEMS Immunol Med Microbiol
JT  - FEMS immunology and medical microbiology
JID - 9315554
RN  - 0 (Complementarity Determining Regions)
RN  - 0 (DNA, Viral)
RN  - 0 (Protein Isoforms)
RN  - 0 (Receptors, Antigen, T-Cell, alpha-beta)
SB  - IM
MH  - Adult
MH  - Amino Acid Sequence
MH  - Complementarity Determining Regions/*genetics/immunology
MH  - DNA, Viral/*immunology
MH  - Female
MH  - *Genes, T-Cell Receptor beta
MH  - Hepatitis B virus/immunology
MH  - Hepatitis B, Chronic/*genetics/immunology/virology
MH  - Humans
MH  - Immunophenotyping
MH  - Leukocytes, Mononuclear/immunology/virology
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Protein Isoforms/genetics/immunology
MH  - Receptors, Antigen, T-Cell, alpha-beta/*genetics/immunology
MH  - T-Lymphocytes/immunology/virology
EDAT- 2012/04/04 06:00
MHDA- 2013/04/30 06:00
CRDT- 2012/04/04 06:00
PHST- 2011/11/04 00:00 [received]
PHST- 2012/03/09 00:00 [revised]
PHST- 2012/03/24 00:00 [accepted]
PHST- 2012/04/04 06:00 [entrez]
PHST- 2012/04/04 06:00 [pubmed]
PHST- 2013/04/30 06:00 [medline]
AID - 10.1111/j.1574-695X.2012.00969.x [doi]
PST - ppublish
SO  - FEMS Immunol Med Microbiol. 2012 Aug;65(3):448-55. doi: 
      10.1111/j.1574-695X.2012.00969.x. Epub 2012 May 21.