PMID- 22469365
OWN - NLM
STAT- MEDLINE
DCOM- 20131101
LR  - 20150317
IS  - 1555-3892 (Electronic)
IS  - 0963-6897 (Linking)
VI  - 21
IP  - 11
DP  - 2012
TI  - Definitive setup of clinical scale procedure for ex vivo expansion of cord blood 
      hematopoietic cells for transplantation.
PG  - 2517-21
LID - 10.3727/096368911X637425 [doi]
AB  - We recently developed a clinical grade ex vivo cord blood expansion procedure 
      enabling a massive amplification of hematopoietic progenitors without any loss of 
      stem cell potential. This procedure, based on day 14 liquid cultures of cord 
      blood CD34(+) cells, in medium Macopharma HP01 and in the presence of stem cell 
      factor (SCF; 100 ng/ml), fms-related tyrosine kinase 3-ligand (Flt-3L; 100 
      ng/ml), megakaryocyte growth and developmental factor (MGDF; 100 ng/ml), and 
      granulocyte colony-stimulating factor (G-CSF; 10 ng/ml) had to be modified due to 
      the commercially unavailability of clinical grade MGDF molecule. So MGDF was 
      replaced by thrombopoietin (TPO) in fivefold lower dose (20 ng/ml), and culture 
      time was reduced to 12 days. That way, a mean expansion fold of 400, 80, and 150 
      was obtained for total cells, CD34(+) cells, and colony-forming cells (CFCs), 
      respectively. This amplification was associated with a slight enhancing effect on 
      stem cells [Scid repopulating cells (SRCs)]. These are the ultimate preclinical 
      modifications of a clinical grade expansion protocol, which is already employed 
      in an ongoing clinical trial.
FAU - Duchez, Pascale
AU  - Duchez P
AD  - Etablissement Francais du Sang Aquitaine-Limousin, 5 Place Amelie Raba Leon, 
      Bordeaux, France.
FAU - Chevaleyre, Jean
AU  - Chevaleyre J
FAU - Vlaski, Marija
AU  - Vlaski M
FAU - Dazey, Bernard
AU  - Dazey B
FAU - Milpied, Noel
AU  - Milpied N
FAU - Boiron, Jean-Michel
AU  - Boiron JM
FAU - Ivanovic, Zoran
AU  - Ivanovic Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120328
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
SB  - IM
MH  - Cells, Cultured
MH  - Fetal Blood/*cytology
MH  - Hematopoietic Stem Cell Transplantation/*methods
MH  - Hematopoietic Stem Cells/cytology
MH  - Humans
EDAT- 2012/04/04 06:00
MHDA- 2013/11/02 06:00
CRDT- 2012/04/04 06:00
PHST- 2012/04/04 06:00 [entrez]
PHST- 2012/04/04 06:00 [pubmed]
PHST- 2013/11/02 06:00 [medline]
AID - ct0536duchez [pii]
AID - 10.3727/096368911X637425 [doi]
PST - ppublish
SO  - Cell Transplant. 2012;21(11):2517-21. doi: 10.3727/096368911X637425. Epub 2012 
      Mar 28.