PMID- 22469747
OWN - NLM
STAT- MEDLINE
DCOM- 20120917
LR  - 20211203
IS  - 1095-9564 (Electronic)
IS  - 1074-7427 (Linking)
VI  - 97
IP  - 4
DP  - 2012 May
TI  - Epigenetic regulation of reelin and brain-derived neurotrophic factor genes in 
      long-term potentiation in rat medial prefrontal cortex.
PG  - 425-40
LID - 10.1016/j.nlm.2012.03.007 [doi]
AB  - Epigenetic mechanisms have recently been known to play fundamental roles in the 
      regulation of synaptic plasticity, and learning and memory tasks in many brain 
      regions, such as the hippocampus, the amygdala, the insular cortex. However, 
      epigenetic mechanism in the medial prefrontal cortex (mPFC), also a crucial 
      neural locus for the control of cognition and emotion, is not well known. The 
      present study investigated the epigenetic regulation of two genes, reelin and 
      brain-derived neurotrophic factor (bdnf), both play important roles in neural 
      plasticity, in the mPFC. The data showed that the levels of total DNA 
      methyltransferase (DNMTs), total histone acetyltransferases (HATs), global 
      acetylated histone 3 (H3) and global acetylated histone 4 (H4) were all changed 
      with the induction of long-term potentiation (LTP) in the mPFC, implying that DNA 
      methylation and histone acetylation may involve in synaptic plasticity in the 
      mPFC. The present results further demonstrated that the demethylation status of 
      reelin and bdnf, and acetylated H3 and acetylated H4 at the reelin and the bdnf 
      promoters in the mPFC were enhanced by the delivery of LTP-inducing 
      high-frequency stimulation (HFS). Consistently, infusion of DNMT inhibitor, 
      5-azacytidine (5-azaC), or histone deacetylases (HDACs) inhibitor, sodium 
      butyrate (NaB), into the mPFC could interfere with LTP-associated demethylation 
      and acetylation of reelin and bdnf genes, and the induction of LTP as well. 
      Long-term retention of trace fear memory, which is dependent on mPFC function, 
      was also altered by administration of these inhibitors into the mPFC. These 
      findings suggest that epigenetic regulation of DNA demethylation and histone 
      acetylation of target genes, such as reelin and bdnf, might underlie the 
      mechanisms of synaptic plasticity and memory retention in the mPFC.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Sui, Li
AU  - Sui L
AD  - School of Medical Instrument and Food Engineering, University of Shanghai for 
      Science and Technology, Shanghai 200093, China. lsui@usst.edu.cn
FAU - Wang, Yan
AU  - Wang Y
FAU - Ju, Li-Hua
AU  - Ju LH
FAU - Chen, Meng
AU  - Chen M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120324
PL  - United States
TA  - Neurobiol Learn Mem
JT  - Neurobiology of learning and memory
JID - 9508166
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cell Adhesion Molecules, Neuronal)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Histones)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Reelin Protein)
RN  - 0 (Reln protein, rat)
RN  - EC 2.1.1.- (DNA Modification Methylases)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
RN  - EC 3.4.21.- (Serine Endopeptidases)
SB  - IM
MH  - Acetylation
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics/metabolism
MH  - Cell Adhesion Molecules, Neuronal/*genetics/metabolism
MH  - DNA Methylation/physiology
MH  - DNA Modification Methylases/analysis
MH  - Epigenesis, Genetic/*physiology
MH  - Extracellular Matrix Proteins/*genetics/metabolism
MH  - Fear/physiology
MH  - Histone Acetyltransferases/analysis
MH  - Histones/analysis
MH  - Long-Term Potentiation/*genetics
MH  - Male
MH  - Memory/*physiology
MH  - Nerve Tissue Proteins/*genetics/metabolism
MH  - Neuronal Plasticity/*genetics
MH  - Prefrontal Cortex/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reelin Protein
MH  - Serine Endopeptidases/*genetics/metabolism
EDAT- 2012/04/04 06:00
MHDA- 2012/09/18 06:00
CRDT- 2012/04/04 06:00
PHST- 2011/10/15 00:00 [received]
PHST- 2012/03/13 00:00 [revised]
PHST- 2012/03/15 00:00 [accepted]
PHST- 2012/04/04 06:00 [entrez]
PHST- 2012/04/04 06:00 [pubmed]
PHST- 2012/09/18 06:00 [medline]
AID - S1074-7427(12)00037-8 [pii]
AID - 10.1016/j.nlm.2012.03.007 [doi]
PST - ppublish
SO  - Neurobiol Learn Mem. 2012 May;97(4):425-40. doi: 10.1016/j.nlm.2012.03.007. Epub 
      2012 Mar 24.