PMID- 22469996
OWN - NLM
STAT- MEDLINE
DCOM- 20120907
LR  - 20211021
IS  - 1096-0309 (Electronic)
IS  - 0003-2697 (Print)
IS  - 0003-2697 (Linking)
VI  - 425
IP  - 2
DP  - 2012 Jun 15
TI  - Human skeletal muscle fiber type specific protein content.
PG  - 175-82
LID - 10.1016/j.ab.2012.03.018 [doi]
AB  - The aim of this project was to develop a method to assess fiber type specific 
      protein content across the continuum of human skeletal muscle fibers. Individual 
      vastus lateralis muscle fibers (n = 264) were clipped into two portions: one for 
      sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) fiber typing 
      and one for Western blot protein identification. Following fiber type 
      determination, fiber segments were combined into fiber type specific pools ( approximately 20 
      fibers/pool) and measured for total protein quantity, glyceraldehyde 3-phosphate 
      dehydrogenase (GAPDH), citrate synthase (CS), and total p38 content. GAPDH 
      content was 64, 54, 160, and 138% more abundant in myosin heavy chain (MHC) 
      I/IIa, MHC IIa, MHC IIa/IIx, and MHC IIx fibers, respectively, when compared with 
      MHC I. Inversely, CS content was 528, 472, 242, and 47% more abundant in MHC I, 
      MHC I/IIa, MHC IIa, and MHC IIa/IIx fibers, respectively, when compared with MHC 
      IIx. Total p38 content was 87% greater in MHC IIa versus MHC I fibers. These data 
      and this approach establish a reliable method for human skeletal muscle fiber 
      type specific protein analysis. Initial results show that particular proteins 
      exist in a hierarchal fashion throughout the continuum of human skeletal muscle 
      fiber types, further highlighting the necessity of fiber type specific analysis.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Galpin, Andrew J
AU  - Galpin AJ
AD  - Human Performance Laboratory, Ball State University, Muncie, IN 47306, USA.
FAU - Raue, Ulrika
AU  - Raue U
FAU - Jemiolo, Bozena
AU  - Jemiolo B
FAU - Trappe, Todd A
AU  - Trappe TA
FAU - Harber, Matthew P
AU  - Harber MP
FAU - Minchev, Kiril
AU  - Minchev K
FAU - Trappe, Scott
AU  - Trappe S
LA  - eng
GR  - R01 AG038576/AG/NIA NIH HHS/United States
GR  - AG038576/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20120330
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 0 (Proteins)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
RN  - EC 2.3.3.1 (Citrate (si)-Synthase)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 3.6.4.1 (Myosin Heavy Chains)
SB  - IM
MH  - *Blotting, Western
MH  - Citrate (si)-Synthase/analysis
MH  - *Electrophoresis, Polyacrylamide Gel
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/analysis
MH  - Humans
MH  - Muscle Fibers, Skeletal/*metabolism
MH  - Myosin Heavy Chains/metabolism
MH  - Proteins/*analysis
MH  - p38 Mitogen-Activated Protein Kinases/analysis
PMC - PMC3358799
MID - NIHMS373857
EDAT- 2012/04/04 06:00
MHDA- 2012/09/08 06:00
PMCR- 2013/06/15
CRDT- 2012/04/04 06:00
PHST- 2011/12/12 00:00 [received]
PHST- 2012/02/28 00:00 [revised]
PHST- 2012/03/22 00:00 [accepted]
PHST- 2012/04/04 06:00 [entrez]
PHST- 2012/04/04 06:00 [pubmed]
PHST- 2012/09/08 06:00 [medline]
PHST- 2013/06/15 00:00 [pmc-release]
AID - S0003-2697(12)00192-3 [pii]
AID - 10.1016/j.ab.2012.03.018 [doi]
PST - ppublish
SO  - Anal Biochem. 2012 Jun 15;425(2):175-82. doi: 10.1016/j.ab.2012.03.018. Epub 2012 
      Mar 30.