PMID- 22470450
OWN - NLM
STAT- MEDLINE
DCOM- 20120803
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 3
DP  - 2012
TI  - Effects of hepatitis B virus S protein exposure on sperm membrane integrity and 
      functions.
PG  - e33471
LID - 10.1371/journal.pone.0033471 [doi]
LID - e33471
AB  - BACKGROUND: Hepatitis B is a public health problem worldwide. Viral infection can 
      affect a man's fertility, but only scant information about the influence of 
      hepatitis B virus (HBV) infection on sperm quality is available. The purpose of 
      this study was to investigate the effect of hepatitis B virus S protein (HBs) on 
      human sperm membrane integrity and functions. METHODS/PRINCIPAL FINDINGS: 
      Reactive oxygen species (ROS), lipid peroxidation (LP), total antioxidant 
      capacity (TAC) and phosphatidylserine (PS) externalization were determined. The 
      terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) 
      assays and flow cytometric analyses were performed. (1) After 3 h incubation with 
      25 microg/ml of HBs, the average rates of ROS positive cells, annexin 
      V-positive/propidium iodide (PI)-negative cells, Caspases-3,-8,-9 positive cells 
      and TUNEL-positive cells were significantly increased in the test groups as 
      compared to those in the control groups, while TAC level was decreased when 
      compared with the control. The level of malondialdehyde (MDA) in the sperm cells 
      exposed to 50 microg/ml of HBs for 3 h was significantly higher than that in the 
      control (P<0.05-0.01). (2) HBs increased the MDA levels and the numbers of ROS 
      positive cells, annexin V-positive/PI-negative cells, caspases-3, -8, -9 positive 
      cells and TUNEL-positive cells in a dose-dependent manner. (3) HBs monoclonal 
      antibody (MAb) and N-Acetylcysteine (NAC) reduced the number of ROS-positive 
      sperm cells. (4) HBs decreased the TAC levels in sperm cells in a dose-dependent 
      manner. CONCLUSION: HBs exposure could lead to ROS generation, lipid 
      peroxidation, TAC reduction, PS externalization, activation of caspases, and DNA 
      fragmentation, resulting in increased apoptosis of sperm cells and loss of sperm 
      membrane integrity and causing sperm dysfunctions.
FAU - Kang, XiangJin
AU  - Kang X
AD  - Research Center for Reproductive Medicine, Shantou University Medical College, 
      Shantou, China.
FAU - Xie, QingDong
AU  - Xie Q
FAU - Zhou, XiaoLing
AU  - Zhou X
FAU - Li, FangZheng
AU  - Li F
FAU - Huang, JiHua
AU  - Huang J
FAU - Liu, DongLing
AU  - Liu D
FAU - Huang, TianHua
AU  - Huang T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120328
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Annexin A5)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Hepatitis B Surface Antigens)
RN  - 0 (Phosphatidylserines)
RN  - 0 (Reactive Oxygen Species)
RN  - 36015-30-2 (Propidium)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspase 8)
RN  - EC 3.4.22.- (Caspase 9)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - Acetylcysteine/pharmacology
MH  - Annexin A5/metabolism
MH  - Antibodies, Monoclonal/immunology/pharmacology
MH  - Apoptosis/drug effects
MH  - Caspase 3/metabolism
MH  - Caspase 8/metabolism
MH  - Caspase 9/metabolism
MH  - Cell Membrane/drug effects
MH  - Chromosomes/metabolism
MH  - DNA Damage/drug effects
MH  - Hepatitis B Surface Antigens/immunology/*pharmacology
MH  - Hepatitis B virus/metabolism
MH  - Humans
MH  - Lipid Peroxidation/drug effects
MH  - Male
MH  - Malondialdehyde/metabolism
MH  - Phosphatidylserines/metabolism
MH  - Propidium/pharmacology
MH  - Reactive Oxygen Species/metabolism
MH  - Spermatozoa/*drug effects/metabolism
PMC - PMC3314651
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2012/04/04 06:00
MHDA- 2012/08/04 06:00
PMCR- 2012/03/28
CRDT- 2012/04/04 06:00
PHST- 2011/11/07 00:00 [received]
PHST- 2012/02/09 00:00 [accepted]
PHST- 2012/04/04 06:00 [entrez]
PHST- 2012/04/04 06:00 [pubmed]
PHST- 2012/08/04 06:00 [medline]
PHST- 2012/03/28 00:00 [pmc-release]
AID - PONE-D-11-22071 [pii]
AID - 10.1371/journal.pone.0033471 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(3):e33471. doi: 10.1371/journal.pone.0033471. Epub 2012 Mar 28.