PMID- 22471470
OWN - NLM
STAT- MEDLINE
DCOM- 20120801
LR  - 20171116
IS  - 2476-762X (Electronic)
IS  - 1513-7368 (Linking)
VI  - 12
IP  - 12
DP  - 2011
TI  - Fenugreek, a naturally occurring edible spice, kills MCF-7 human breast cancer 
      cells via an apoptotic pathway.
PG  - 3299-304
AB  - There is growing use of anticancer complementary and alternative medicines 
      worldwide. Trigonella foenum graecum (Fenugreek) is traditionally applied to 
      treat disorders such as diabetes, high cholesterol, wounds, inflammation, and 
      gastrointestinal ailments. Fenugreek is also reported to have anticancer 
      properties due to its active beneficial chemical constituents. The mechanism of 
      action of several anticancer drugs is based on their ability to induce apoptosis. 
      The objective of the study was to characterize the downstream apoptotic genes 
      targeted by FCE in MCF-7 human immortalized breast cells. FCE effectively killed 
      MCF-7 cells through induction of apoptosis,confirmed by terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) and RT-PCR assays. When cells 
      were exposed to 50 mug/mL FCE for 24 hours, 23.2% apoptotic cells resulted, while 
      a 48-hour exposure to 50 mug/mL caused 73.8% apoptosis. This was associated with 
      increased expression of Caspase 3, 8, 9, p53, Fas, FADD, Bax and Bak in a 
      time-and dose-dependent manner, as determined by real- time quantitative PCR. In 
      summary, the induction of apoptosis by FCE is effected by its ability to increase 
      the expression of pro-apoptotic genes and the spice holds promise for 
      consideration in complementary therapy for breast cancer patients.
FAU - Khoja, Kholoud K
AU  - Khoja KK
AD  - Dept. of Food Sciences and Nutrition, King Saud University, Riyadh, Saudi Arabia.
FAU - Shaf, Gowhar
AU  - Shaf G
FAU - Hasan, Tarique N
AU  - Hasan TN
FAU - Syed, Naveed Ahmed
AU  - Syed NA
FAU - Al-Khalifa, Abdrohman S
AU  - Al-Khalifa AS
FAU - Al-Assaf, Abdullah H
AU  - Al-Assaf AH
FAU - Alshatwi, Ali A
AU  - Alshatwi AA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Thailand
TA  - Asian Pac J Cancer Prev
JT  - Asian Pacific journal of cancer prevention : APJCP
JID - 101130625
RN  - 0 (BAK1 protein, human)
RN  - 0 (BAX protein, human)
RN  - 0 (FADD protein, human)
RN  - 0 (FAS protein, human)
RN  - 0 (Fas-Associated Death Domain Protein)
RN  - 0 (Plant Extracts)
RN  - 0 (RNA, Messenger)
RN  - 0 (TP53 protein, human)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 0 (bcl-2 Homologous Antagonist-Killer Protein)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 0 (fas Receptor)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Breast Neoplasms/*drug therapy/metabolism/*pathology
MH  - Caspases/genetics/metabolism
MH  - Fas-Associated Death Domain Protein/genetics/metabolism
MH  - Female
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - *Phytotherapy
MH  - Plant Extracts/*therapeutic use
MH  - RNA, Messenger/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Trigonella/*chemistry
MH  - Tumor Cells, Cultured
MH  - Tumor Suppressor Protein p53/genetics/metabolism
MH  - bcl-2 Homologous Antagonist-Killer Protein/genetics/metabolism
MH  - bcl-2-Associated X Protein/genetics/metabolism
MH  - fas Receptor/genetics/metabolism
EDAT- 2011/01/01 00:00
MHDA- 2012/08/02 06:00
CRDT- 2012/04/05 06:00
PHST- 2012/04/05 06:00 [entrez]
PHST- 2011/01/01 00:00 [pubmed]
PHST- 2012/08/02 06:00 [medline]
PST - ppublish
SO  - Asian Pac J Cancer Prev. 2011;12(12):3299-304.