PMID- 22471607
OWN - NLM
STAT- MEDLINE
DCOM- 20121001
LR  - 20120404
IS  - 1532-4230 (Electronic)
IS  - 1532-1819 (Linking)
VI  - 33
IP  - 2
DP  - 2012
TI  - Detection of adiponectin and monocyte chemoattractant protein-1 using a 
      calixcrown derivatives-coated protein chip.
PG  - 166-79
LID - 10.1080/15321819.2011.615360 [doi]
AB  - We used a ProteoChip coated with a calixcrown derivative protein linker to 
      measure adiponectin and monocyte chemoattractant protein-1 (MCP-1) levels and 
      compared the results with commercial enzyme-linked immunosorbent assay (ELISA) 
      kits. Adiponectin and MCP-1 levels in normal human serum and RAW264 cell 
      supernatants, respectively, were measured. The ProteoChip quantification results 
      correlated with those from the ELISA kits; however, the ProteoChip required less 
      sample volume, exhibited higher sensitivity, and had a wider detection range. The 
      ProteoChip was capable of detecting and quantifying small amounts of protein, 
      possibly replacing ELISA kits in evaluating the levels of adiponectin and MCP-1.
FAU - Tanabe, Rima
AU  - Tanabe R
AD  - Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
FAU - Cha, Byung-Yoon
AU  - Cha BY
FAU - Ha, Byung Geun
AU  - Ha BG
FAU - Yonezawa, Takayuki
AU  - Yonezawa T
FAU - Nagai, Kazuo
AU  - Nagai K
FAU - Woo, Je-Tae
AU  - Woo JT
FAU - Yagasaki, Kazumi
AU  - Yagasaki K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Immunoassay Immunochem
JT  - Journal of immunoassay & immunochemistry
JID - 100963688
RN  - 0 (Adiponectin)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Crown Ethers)
RN  - 0 (Recombinant Proteins)
RN  - 130036-26-9 (Calixarenes)
SB  - IM
MH  - Adiponectin/*analysis/immunology
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Calixarenes/*chemistry/immunology
MH  - Cell Line
MH  - Chemokine CCL2/*analysis/immunology
MH  - Crown Ethers/*chemistry/immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Mice
MH  - Protein Array Analysis/instrumentation/*methods
MH  - Recombinant Proteins/analysis/immunology
MH  - Sensitivity and Specificity
EDAT- 2012/04/05 06:00
MHDA- 2012/10/02 06:00
CRDT- 2012/04/05 06:00
PHST- 2012/04/05 06:00 [entrez]
PHST- 2012/04/05 06:00 [pubmed]
PHST- 2012/10/02 06:00 [medline]
AID - 10.1080/15321819.2011.615360 [doi]
PST - ppublish
SO  - J Immunoassay Immunochem. 2012;33(2):166-79. doi: 10.1080/15321819.2011.615360.