PMID- 22471805
OWN - NLM
STAT- MEDLINE
DCOM- 20120614
LR  - 20120404
IS  - 1095-8649 (Electronic)
IS  - 0022-1112 (Linking)
VI  - 80
IP  - 4
DP  - 2012 Apr
TI  - Characterization of yellow grouper Epinephelus awoara (Serranidae) karyotype by 
      chromosome bandings and fluorescence in situ hybridization.
PG  - 866-75
LID - 10.1111/j.1095-8649.2012.03230.x [doi]
AB  - The cytogenetics of yellow grouper Epinephelus awoara was studied using multiple 
      cytogenetic markers [Giemsa staining, C-banding, Ag-NORs and fluorescence in situ 
      hybridization (FISH)]. Giemsa staining results showed that the karyotypic formula 
      of E. awoara was 2n = 48a, FN (fundamental number) = 48. Faint C-bandings were 
      only detected at the centromeric regions of chromosome pair number 24, being 
      almost indiscernible on the other chromosome pairs. After Ag-NOR staining, one 
      pair of nucleolar organizer regions (NOR) was observed in the subcentromeric 
      region of pair number 24. FISH results showed that 5S rDNA was located at a pair 
      of medium-sized chromosomes, while 18S rDNA appeared at the same location in the 
      subcentromeric region of pair number 24 where Ag-NORs were detected. The 
      telomeric sequence (TTAGGG)(n) detected by FISH was located at both ends of each 
      chromosome. The results suggested that E. awoara has retained general karyotypic 
      structure stability during the evolutionary diversification process.
CI  - (c) 2012 The Authors. Journal of Fish Biology (c) 2012 The Fisheries Society of the 
      British Isles.
FAU - Wang, S F
AU  - Wang SF
AD  - Key Laboratory of Tropical Biological Resources of Ministry of Education, Hainan 
      University, Haikou 570228, China.
FAU - Cai, Y
AU  - Cai Y
FAU - Qin, Y X
AU  - Qin YX
FAU - Zhou, Y C
AU  - Zhou YC
FAU - Su, Y Q
AU  - Su YQ
FAU - Wang, J
AU  - Wang J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120305
PL  - England
TA  - J Fish Biol
JT  - Journal of fish biology
JID - 0214055
RN  - 0 (Heterochromatin)
RN  - 0 (RNA, Ribosomal, 18S)
RN  - 0 (RNA, Ribosomal, 5S)
SB  - IM
MH  - Animals
MH  - Bass/*genetics
MH  - Chromosome Banding/*veterinary
MH  - Chromosomes/genetics/metabolism
MH  - Cytogenetic Analysis/veterinary
MH  - Heterochromatin/metabolism
MH  - In Situ Hybridization, Fluorescence/*veterinary
MH  - *Karyotype
MH  - RNA, Ribosomal, 18S/genetics
MH  - RNA, Ribosomal, 5S/genetics
MH  - Terminal Repeat Sequences/genetics
EDAT- 2012/04/05 06:00
MHDA- 2012/06/15 06:00
CRDT- 2012/04/05 06:00
PHST- 2012/04/05 06:00 [entrez]
PHST- 2012/04/05 06:00 [pubmed]
PHST- 2012/06/15 06:00 [medline]
AID - 10.1111/j.1095-8649.2012.03230.x [doi]
PST - ppublish
SO  - J Fish Biol. 2012 Apr;80(4):866-75. doi: 10.1111/j.1095-8649.2012.03230.x. Epub 
      2012 Mar 5.