PMID- 22471939
OWN - NLM
STAT- MEDLINE
DCOM- 20130419
LR  - 20211021
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Print)
IS  - 1547-3287 (Linking)
VI  - 21
IP  - 17
DP  - 2012 Nov 20
TI  - Human olfactory mucosa multipotent mesenchymal stromal cells promote survival, 
      proliferation, and differentiation of human hematopoietic cells.
PG  - 3187-96
LID - 10.1089/scd.2012.0084 [doi]
AB  - Multipotent mesenchymal stromal cells (MSCs) from the human olfactory mucosa (OM) 
      are cells that have been proposed as a niche for neural progenitors. OM-MSCs 
      share phenotypic and functional properties with bone marrow (BM) MSCs, which 
      constitute fundamental components of the hematopoietic niche. In this work, we 
      investigated whether human OM-MSCs may promote the survival, proliferation, and 
      differentiation of human hematopoietic stem cells (HSCs). For this purpose, human 
      bone marrow cells (BMCs) were co-cultured with OM-MSCs in the absence of 
      exogenous cytokines. At different intervals, nonadherent cells (NACs) were 
      harvested from BMC/OM-MSC co-cultures, and examined for the expression of blood 
      cell markers by flow cytometry. OM-MSCs supported the survival (cell viability 
      >90%) and proliferation of BMCs, after 54 days of co-culture. At 20 days of 
      co-culture, flow cytometric and microscopic analyses showed a high percentage 
      (73%) of cells expressing the pan-leukocyte marker CD45, and the presence of 
      cells of myeloid origin, including polymorphonuclear leukocytes, monocytes, 
      basophils, eosinophils, erythroid cells, and megakaryocytes. Likewise, T (CD3), B 
      (CD19), and NK (CD56/CD16) cells were detected in the NAC fraction. 
      Colony-forming unit-granulocyte/macrophage (CFU-GM) progenitors and CD34(+) cells 
      were found, at 43 days of co-culture. Reverse transcriptase-polymerase chain 
      reaction (RT-PCR) studies showed that OM-MSCs constitutively express early and 
      late-acting hematopoietic cytokines (i.e., stem cell factor [SCF] and 
      granulocyte- macrophage colony-stimulating factor [GM-CSF]). These results 
      constitute the first evidence that OM-MSCs may provide an in vitro 
      microenvironment for HSCs. The capacity of OM-MSCs to support the survival and 
      differentiation of HSCs may be related with the capacity of OM-MSCs to produce 
      hematopoietic cytokines.
FAU - Diaz-Solano, Dylana
AU  - Diaz-Solano D
AD  - Unidad de Terapia Celular-Laboratorio de Patologia Celular y Molecular, Centro de 
      Medicina Experimental, Instituto Venezolano de Investigaciones Cientificas 
      (IVIC), Caracas, Venezuela.
FAU - Wittig, Olga
AU  - Wittig O
FAU - Ayala-Grosso, Carlos
AU  - Ayala-Grosso C
FAU - Pieruzzini, Rosalinda
AU  - Pieruzzini R
FAU - Cardier, Jose E
AU  - Cardier JE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120518
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 0 (Antigens, CD34)
RN  - 0 (Biomarkers)
RN  - 0 (CD56 Antigen)
RN  - 0 (NCAM1 protein, human)
RN  - 0 (Stem Cell Factor)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
RN  - EC 3.1.3.48 (PTPRC protein, human)
SB  - IM
MH  - Antigens, CD34/genetics/metabolism
MH  - Biomarkers/metabolism
MH  - Bone Marrow Cells/cytology/metabolism
MH  - CD56 Antigen/genetics/metabolism
MH  - Cell Count
MH  - Cell Culture Techniques/methods
MH  - *Cell Differentiation
MH  - *Cell Proliferation
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Cellular Microenvironment
MH  - Coculture Techniques/methods
MH  - Colony-Forming Units Assay
MH  - Flow Cytometry
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/genetics/metabolism
MH  - Hematopoietic Stem Cells/*cytology/metabolism
MH  - Humans
MH  - Leukocyte Common Antigens/genetics/metabolism
MH  - Leukocytes, Mononuclear/metabolism
MH  - Lymphopoiesis
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Myelopoiesis
MH  - Olfactory Mucosa/*cytology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stem Cell Factor/genetics/metabolism
MH  - Time Factors
PMC - PMC3495125
EDAT- 2012/04/05 06:00
MHDA- 2013/04/23 06:00
PMCR- 2013/11/20
CRDT- 2012/04/05 06:00
PHST- 2012/04/05 06:00 [entrez]
PHST- 2012/04/05 06:00 [pubmed]
PHST- 2013/04/23 06:00 [medline]
PHST- 2013/11/20 00:00 [pmc-release]
AID - 10.1089/scd.2012.0084 [pii]
AID - 10.1089/scd.2012.0084 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2012 Nov 20;21(17):3187-96. doi: 10.1089/scd.2012.0084. Epub 2012 
      May 18.