PMID- 22472192
OWN - NLM
STAT- MEDLINE
DCOM- 20121113
LR  - 20131121
IS  - 1096-0929 (Electronic)
IS  - 1096-0929 (Linking)
VI  - 128
IP  - 1
DP  - 2012 Jul
TI  - Analysis of leukemia-specific aneuploidies in cultured myeloid progenitor cells 
      in the absence and presence of formaldehyde exposure.
PG  - 72-8
LID - 10.1093/toxsci/kfs126 [doi]
AB  - A recently published human study suggested that exposure to formaldehyde (FA) at 
      the workplace might induce leukemia-specific aneuploidies (monosomy 7 and trisomy 
      8) in cultured myeloid progenitor cells. Despite its preliminary character, this 
      study was considered by the International Agency for Research on Cancer to be a 
      potential mechanistic explanation for the induction of leukemia by FA. To further 
      evaluate the reliability of these findings, chromosome preparations from cultured 
      myeloid progenitor cells (obtained from blood samples of five healthy subjects) 
      were analyzed by fluorescence in situ hybridization (FISH) for spontaneously 
      occurring numerical aberrations after cultivation for 9 days. FISH analysis with 
      probes for chromosomes 6, 7, and 8 revealed that the baseline frequency of 
      aneuploid metaphases is similar and rather low for all three chromosomes tested. 
      More monosomies than trisomies were measured. We also exposed myeloid progenitor 
      cells during the whole cultivation period to FA and determined the frequency of 
      aneuploidies after 9 days of cultivation. The results clearly indicate that FA 
      did not induce aneuploidy under these experimental conditions. In contrast, 
      aneuploidy was induced under these conditions by the known aneugen vincristine. 
      Myeloid progenitor cells from healthy subjects were not particularly sensitive 
      toward the cytotoxic action of FA. Colony forming ability in the presence of FA 
      was not reduced to a higher degree than in cultured cell lines (A549; V79). Our 
      results do not support the assumption of a specific effect of FA on myeloid 
      progenitor cells as a potential mechanism for the induction of leukemia.
FAU - Kuehner, Stefanie
AU  - Kuehner S
AD  - Institut fur Humangenetik, Universitat Ulm, D-89081 Ulm, Germany.
FAU - Schlaier, Martina
AU  - Schlaier M
FAU - Schwarz, Klaus
AU  - Schwarz K
FAU - Speit, Gunter
AU  - Speit G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120403
PL  - United States
TA  - Toxicol Sci
JT  - Toxicological sciences : an official journal of the Society of Toxicology
JID - 9805461
RN  - 1HG84L3525 (Formaldehyde)
SB  - IM
MH  - *Aneuploidy
MH  - Cells, Cultured
MH  - Formaldehyde/*toxicity
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia/*genetics/pathology
MH  - Myeloid Progenitor Cells/cytology/*drug effects
EDAT- 2012/04/05 06:00
MHDA- 2012/11/14 06:00
CRDT- 2012/04/05 06:00
PHST- 2012/04/05 06:00 [entrez]
PHST- 2012/04/05 06:00 [pubmed]
PHST- 2012/11/14 06:00 [medline]
AID - kfs126 [pii]
AID - 10.1093/toxsci/kfs126 [doi]
PST - ppublish
SO  - Toxicol Sci. 2012 Jul;128(1):72-8. doi: 10.1093/toxsci/kfs126. Epub 2012 Apr 3.