PMID- 22472558
OWN - NLM
STAT- MEDLINE
DCOM- 20130501
LR  - 20220129
IS  - 1555-3892 (Electronic)
IS  - 0963-6897 (Print)
IS  - 0963-6897 (Linking)
VI  - 21
IP  - 6
DP  - 2012
TI  - Isolation of myogenic stem cells from cultures of cryopreserved human skeletal 
      muscle.
PG  - 1087-93
LID - 10.3727/096368912X636876 [doi]
AB  - We demonstrate that subpopulations of adult human skeletal muscle-derived stem 
      cells, myogenic endothelial cells (MECs), and perivascular stem cells (PSCs) can 
      be simultaneously purified by fluorescence-activated cell sorting (FACS) from 
      cryopreserved human primary skeletal muscle cell cultures (cryo-hPSMCs). For FACS 
      isolation, we utilized a combination of cell lineage markers: the myogenic cell 
      marker CD56, the endothelial cell marker UEA-1 receptor (UEA-1R), and the 
      perivascular cell marker CD146. MECs expressing all three cell lineage markers 
      (CD56(+)UEA-1R(+)CD146(+)/CD45(-)) and PSCs expressing only CD146 
      (CD146(+)/CD45(-)CD56(-)UEA-1R(-)) were isolated by FACS. To evaluate their 
      myogenic capacities, the sorted cells, with and without expansion in culture, 
      were transplanted into the cardiotoxin-injured skeletal muscles of 
      immunodeficient mice. The purified MECs exhibited the highest regenerative 
      capacity in the injured mouse muscles among all cell fractions tested, while PSCs 
      remained superior to myoblasts and the unpurified primary skeletal muscle cells. 
      Our findings show that both MECs and PSCs retain their high myogenic potentials 
      after in vitro expansion, cryopreservation, and FACS sorting. The current study 
      demonstrates that myogenic stem cells are prospectively isolatable from long-term 
      cryopreserved primary skeletal muscle cell cultures. We emphasize the potential 
      application of this new approach to extract therapeutic stem cells from human 
      muscle cells cryogenically banked for clinical purposes.
FAU - Zheng, Bo
AU  - Zheng B
AD  - Stem Cell Research Center, Department of Orthopaedic Surgery, University of 
      Pittsburgh School of Medicine and Children's Hospital of Pittsburgh of UPMC, 
      Pittsburgh, PA, USA.
FAU - Chen, Chien-Wen
AU  - Chen CW
FAU - Li, Guangheng
AU  - Li G
FAU - Thompson, Seth D
AU  - Thompson SD
FAU - Poddar, Minakshi
AU  - Poddar M
FAU - Peault, Bruno
AU  - Peault B
FAU - Huard, Johnny
AU  - Huard J
LA  - eng
GR  - R21 HL083057-01A2/HL/NHLBI NIH HHS/United States
GR  - G1000816/MRC_/Medical Research Council/United Kingdom
GR  - R01DE013420-09/DE/NIDCR NIH HHS/United States
GR  - R21 HL083057/HL/NHLBI NIH HHS/United States
GR  - R01 DE013420/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20120402
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
RN  - 0 (CD146 Antigen)
RN  - 0 (CD56 Antigen)
RN  - 0 (Transcription Factors)
RN  - EC 2.3.2.23 (UBE2V1 protein, human)
RN  - EC 2.3.2.23 (Ubiquitin-Conjugating Enzymes)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Animals
MH  - CD146 Antigen/metabolism
MH  - CD56 Antigen/metabolism
MH  - Cell Differentiation
MH  - Cell Lineage
MH  - Cells, Cultured
MH  - Child
MH  - Child, Preschool
MH  - Cryopreservation
MH  - Endothelial Cells/cytology
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mice, SCID
MH  - Middle Aged
MH  - Muscle, Skeletal/*cytology
MH  - Regeneration/physiology
MH  - Stem Cell Transplantation
MH  - Stem Cells/*cytology/metabolism
MH  - Transcription Factors/metabolism
MH  - Ubiquitin-Conjugating Enzymes/metabolism
MH  - Young Adult
PMC - PMC4356195
MID - NIHMS669167
EDAT- 2012/04/05 06:00
MHDA- 2013/05/02 06:00
PMCR- 2015/03/11
CRDT- 2012/04/05 06:00
PHST- 2012/04/05 06:00 [entrez]
PHST- 2012/04/05 06:00 [pubmed]
PHST- 2013/05/02 06:00 [medline]
PHST- 2015/03/11 00:00 [pmc-release]
AID - ct0204zheng [pii]
AID - 10.3727/096368912X636876 [doi]
PST - ppublish
SO  - Cell Transplant. 2012;21(6):1087-93. doi: 10.3727/096368912X636876. Epub 2012 Apr 
      2.