PMID- 22472731
OWN - NLM
STAT- MEDLINE
DCOM- 20120905
LR  - 20191210
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 166
IP  - 1-2
DP  - 2012 Jun
TI  - Activation of c-Jun NH(2)-terminal kinase is required for porcine reproductive 
      and respiratory syndrome virus-induced apoptosis but not for virus replication.
PG  - 103-8
LID - 10.1016/j.virusres.2012.03.010 [doi]
AB  - Apoptosis of host cells plays a critical role in pathogenesis of virus infection. 
      MAPK kinases especially stress-activated protein kinases c-Jun NH(2)-terminal 
      kinase (SAPK/JNK) and p38 are often involved in virus-mediated apoptosis. It has 
      been shown that porcine reproductive and respiratory syndrome virus (PRRSV) 
      infection resulted in apoptosis of the host cells both in vitro and in vivo. The 
      current investigation was initiated to determine whether stress-activated protein 
      kinases JNK and p38 play a role in apoptosis induction by PRRSV infection. We 
      examined phosphorylation of JNK and p38, and found that JNK but not p38 was 
      activated in response to PRRSV infection. We then examined effects of this kinase 
      on apoptosis induction and virus replication by using specific inhibitor. We 
      found that JNK inhibition by its inhibitor SP600125 led to the abolishment of 
      PRRSV-mediated apoptosis, but did not suppress virus replication. Further studies 
      demonstrated that ROS generation was involved in JNK activation, and Bcl-2 family 
      anti-apoptotic proteins Mcl-1 and Bcl-xl were downstream targets of JNK to 
      mediate apoptosis. We conclude that activation of JNK signaling pathway is 
      essential for PRRSV-mediated apoptosis but not for virus replication.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Yin, Shutao
AU  - Yin S
AD  - Division of Nutrition and Health, College of Food Science and Nutritional 
      Engineering, China Agricultural University, No. 17 Qinghua East Road, Haidian 
      District, Beijing 100083, China.
FAU - Huo, Yazhen
AU  - Huo Y
FAU - Dong, Yinhui
AU  - Dong Y
FAU - Fan, Lihong
AU  - Fan L
FAU - Yang, Hanchun
AU  - Yang H
FAU - Wang, Leyuan
AU  - Wang L
FAU - Ning, Yibao
AU  - Ning Y
FAU - Hu, Hongbo
AU  - Hu H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120327
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - *Host-Pathogen Interactions
MH  - JNK Mitogen-Activated Protein Kinases/*metabolism
MH  - MAP Kinase Signaling System
MH  - Porcine respiratory and reproductive syndrome virus/*pathogenicity/physiology
MH  - *Transcriptional Activation
MH  - *Virus Replication
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
EDAT- 2012/04/05 06:00
MHDA- 2012/09/06 06:00
CRDT- 2012/04/05 06:00
PHST- 2011/11/18 00:00 [received]
PHST- 2012/03/09 00:00 [revised]
PHST- 2012/03/13 00:00 [accepted]
PHST- 2012/04/05 06:00 [entrez]
PHST- 2012/04/05 06:00 [pubmed]
PHST- 2012/09/06 06:00 [medline]
AID - S0168-1702(12)00102-5 [pii]
AID - 10.1016/j.virusres.2012.03.010 [doi]
PST - ppublish
SO  - Virus Res. 2012 Jun;166(1-2):103-8. doi: 10.1016/j.virusres.2012.03.010. Epub 
      2012 Mar 27.