PMID- 22472781
OWN - NLM
STAT- MEDLINE
DCOM- 20150409
LR  - 20181023
IS  - 0048-7449 (Print)
IS  - 0048-7449 (Linking)
VI  - 64
IP  - 1
DP  - 2012 Mar 19
TI  - In vitro effect of anti-beta(2) glycoprotein I antibodies on P-selectin expression, a 
      marker of platelet activation.
PG  - 35-9
LID - 10.4081/reumatismo.2012.35 [doi]
AB  - OBJECTIVE: Antiphospholipid antibodies (aPL) associated with thrombembolic events 
      and/or pregnancy morbidity characterize the so-called antiphospholipid syndrome 
      (APS). Beta2glycoprotein I (beta2GPI) represents the major target antigen for aPL, 
      but the pathogenic role of anti-beta2GPI antibodies (abeta2GPI) is still unclear. Some 
      authors assume they play a role in activating platelets. The effects of abeta2GPI 
      antibodies on platelet P-selectin expression were evaluated in this study. 
      METHODS: Abeta2GPI antibodies in the plasma of a pregnant APS patient were isolated 
      by affinity chromatography during two different stages (catastrophic and 
      quiescent) of the disease. Gel filtered platelets (100,000/mul) from healthy 
      volunteers were incubated with beta2-GPI (20 mug/ml) and with different 
      concentrations (5, 25 e 50 mug/ml) of abeta2GPI antibodies. P-selectin surface 
      expression on platelets was assessed by flow cytometry using a specific 
      fluorescent antibody directed against P-selectin. RESULTS: Abeta2GPI antibodies 
      induced platelet activation only in the presence of thrombin receptor activator 
      for peptide 6 (TRAP-6), a platelet agonist, at a subthreshold concentration. 
      Abeta2GPI antibody enhancement on platelet surface P-selectin expression was 
      stronger in the catastrophic than in the quiescent phase of the disease (47% 
      versus 15%). CONCLUSIONS: TRAP-6 dependent platelet activation by abeta2GPI 
      antibodies is consistent with the "two hit" pathogenetic hypothesis for 
      thrombosis. Abeta2GPI antibodies induce higher platelet P-selectin expression during 
      the active rather than in the acute phases.
FAU - Bontadi, A
AU  - Bontadi A
AD  - Cattedra e U.O.C. di Reumatologia, Dipartimento di Medicina Clinica e 
      Sperimentale, Universita di Padova, Italy. agnese.bontadi@unipd.it
FAU - Ruffatti, A
AU  - Ruffatti A
FAU - Giannini, S
AU  - Giannini S
FAU - Falcinelli, E
AU  - Falcinelli E
FAU - Tonello, M
AU  - Tonello M
FAU - Hoxha, A
AU  - Hoxha A
FAU - Gresele, P
AU  - Gresele P
FAU - Punzi, L
AU  - Punzi L
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20120319
PL  - Italy
TA  - Reumatismo
JT  - Reumatismo
JID - 0401302
RN  - 0 (Autoantibodies)
RN  - 0 (Autoantigens)
RN  - 0 (P-Selectin)
RN  - 0 (Peptide Fragments)
RN  - 0 (beta 2-Glycoprotein I)
RN  - 0 (thrombin receptor peptide (42-47))
SB  - IM
MH  - Acute Disease
MH  - Adult
MH  - Antiphospholipid Syndrome/*blood/complications/immunology
MH  - Autoantibodies/immunology/isolation & purification/*pharmacology
MH  - Autoantigens/*immunology/isolation & purification
MH  - Chromatography, Affinity
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - In Vitro Techniques
MH  - P-Selectin/*biosynthesis/genetics
MH  - Peptide Fragments/pharmacology
MH  - *Platelet Activation
MH  - Pregnancy
MH  - Pregnancy Complications/*blood/immunology
MH  - Pregnancy Complications, Hematologic/blood/etiology/immunology
MH  - Thrombophilia/blood/*etiology/immunology
MH  - beta 2-Glycoprotein I/*immunology/isolation & purification/pharmacology
EDAT- 2012/04/05 06:00
MHDA- 2015/04/10 06:00
CRDT- 2012/04/05 06:00
PHST- 2011/11/21 00:00 [received]
PHST- 2011/11/30 00:00 [accepted]
PHST- 2012/04/05 06:00 [entrez]
PHST- 2012/04/05 06:00 [pubmed]
PHST- 2015/04/10 06:00 [medline]
AID - reumatismo.2012.35 [pii]
AID - 10.4081/reumatismo.2012.35 [doi]
PST - epublish
SO  - Reumatismo. 2012 Mar 19;64(1):35-9. doi: 10.4081/reumatismo.2012.35.