PMID- 22473786
OWN - NLM
STAT- MEDLINE
DCOM- 20141024
LR  - 20211021
IS  - 1932-7005 (Electronic)
IS  - 1932-6254 (Print)
IS  - 1932-6254 (Linking)
VI  - 7
IP  - 12
DP  - 2013 Dec
TI  - The effect of fresh bone marrow cells on reconstruction of mouse calvarial defect 
      combined with calvarial osteoprogenitor cells and collagen-apatite scaffold.
PG  - 974-83
LID - 10.1002/term.1490 [doi]
AB  - Fresh bone marrow cells have already exhibited its advantages as osteogenic donor 
      cells, but the combination between fresh bone marrow cells and other donor cells 
      utilized for bone healing has not been fully explored. To highlight the impact of 
      fresh bone marrow cells on scaffold-based bone regeneration, single or a 
      combination of calvarial osteoprogenitor cells (OPCs) and bone marrow cells 
      (BMCs) were used as donor cells combined with collagen-apatite scaffold for 
      calvarial defect healing. The host and donor contributions to bone formation were 
      assessed using histological and GFP imaging analysis. Although the amount of new 
      bone formed by different cell sources did not show significant differences, the 
      origin of the bone formation in the defects mainly depended on the types of donor 
      cells employed: when only calvarial OPCs were used as donor cells, a 
      donor-derived bone healing instead of host-derived bone ingrowth was observed; 
      when only fresh BMCs were loaded, the host bone could grow into the defect along 
      the lamellar structure of the scaffolds, but the amount of new bone formed was 
      significantly lower than the defect loaded with calvarial OPCs only. The 
      combination of calvarial OPCs and fresh BMCs had similar amount of new bone 
      formation as the group loaded with calvarial osteoprogenitors alone, but did not 
      induce any host-derived bone formation. These results provide compelling evidence 
      of the importance of fresh BMCs to induce host-implant integration in bone tissue 
      engineering.
CI  - Copyright (c) 2012 John Wiley & Sons, Ltd.
FAU - Yu, Xiaohua
AU  - Yu X
AD  - Department of Chemical, Materials and Biomolecular Engineering, University of 
      Connecticut, Storrs, CT, 06269, USA.
FAU - Wang, Liping
AU  - Wang L
FAU - Peng, Fei
AU  - Peng F
FAU - Jiang, Xi
AU  - Jiang X
FAU - Xia, Zengmin
AU  - Xia Z
FAU - Huang, Jianping
AU  - Huang J
FAU - Rowe, David
AU  - Rowe D
FAU - Wei, Mei
AU  - Wei M
LA  - eng
GR  - R21 AR059962/AR/NIAMS NIH HHS/United States
GR  - 1R21AR059962-01A1/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20120331
PL  - England
TA  - J Tissue Eng Regen Med
JT  - Journal of tissue engineering and regenerative medicine
JID - 101308490
RN  - 0 (Apatites)
RN  - 0 (Isoenzymes)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 9007-34-5 (Collagen)
RN  - EC 3.1.3.2 (Acid Phosphatase)
RN  - EC 3.1.3.2 (Tartrate-Resistant Acid Phosphatase)
SB  - IM
MH  - Acid Phosphatase/metabolism
MH  - Animals
MH  - Apatites/*pharmacology
MH  - Bone Marrow Cells/*cytology/drug effects
MH  - Bone Marrow Transplantation
MH  - Collagen/*pharmacology
MH  - Disease Models, Animal
MH  - Green Fluorescent Proteins/metabolism
MH  - Isoenzymes/metabolism
MH  - Mice
MH  - Osteoclasts/cytology/drug effects
MH  - Prosthesis Implantation
MH  - Rats
MH  - Regenerative Medicine/*methods
MH  - Skull/diagnostic imaging/*pathology
MH  - Staining and Labeling
MH  - Stem Cell Transplantation
MH  - Stem Cells/*cytology/drug effects
MH  - Tartrate-Resistant Acid Phosphatase
MH  - Tissue Scaffolds/*chemistry
MH  - X-Ray Microtomography
PMC - PMC4053253
MID - NIHMS593620
OTO - NOTNLM
OT  - bone tissue engineering
OT  - fresh bone marrow cells
OT  - green fluorescence protein
OT  - host integration
OT  - osteoblasts
EDAT- 2012/04/05 06:00
MHDA- 2014/10/25 06:00
PMCR- 2014/12/01
CRDT- 2012/04/05 06:00
PHST- 2011/03/10 00:00 [received]
PHST- 2012/01/10 00:00 [revised]
PHST- 2012/01/19 00:00 [accepted]
PHST- 2012/04/05 06:00 [entrez]
PHST- 2012/04/05 06:00 [pubmed]
PHST- 2014/10/25 06:00 [medline]
PHST- 2014/12/01 00:00 [pmc-release]
AID - 10.1002/term.1490 [doi]
PST - ppublish
SO  - J Tissue Eng Regen Med. 2013 Dec;7(12):974-83. doi: 10.1002/term.1490. Epub 2012 
      Mar 31.