PMID- 22474216
OWN - NLM
STAT- MEDLINE
DCOM- 20121009
LR  - 20211021
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Print)
IS  - 0032-0889 (Linking)
VI  - 159
IP  - 2
DP  - 2012 Jun
TI  - The helicase and RNaseIIIa domains of Arabidopsis Dicer-Like1 modulate catalytic 
      parameters during microRNA biogenesis.
PG  - 748-58
LID - 10.1104/pp.112.193508 [doi]
AB  - Dicer-Like1 (DCL1), an RNaseIII endonuclease, and Hyponastic Leaves1 (HYL1), a 
      double-stranded RNA-binding protein, are core components of the plant microRNA 
      (miRNA) biogenesis machinery. hyl1 null mutants accumulate low levels of miRNAs 
      and display pleiotropic developmental phenotypes. We report the identification of 
      five new hyl1 suppressor mutants, all of which are alleles of DCL1. These new 
      alleles affect either the helicase or the RNaseIIIa domains of DCL1, highlighting 
      the critical functions of these domains. Biochemical analysis of the DCL1 
      suppressor variants reveals that they process the primary transcript (pri-miRNA) 
      more efficiently than wild-type DCL1, with both higher K(cat) and lower K(m) 
      values. The DCL1 variants largely rescue wild-type miRNA accumulation levels in 
      vivo, but do not rescue the MIRNA processing precision defects of the hyl1 null 
      mutant. In vitro, the helicase domain confers ATP dependence on DCL1-catalyzed 
      MIRNA processing, attenuates DCL1 cleavage activity, and is required for precise 
      MIRNA processing of some substrates.
FAU - Liu, Chenggang
AU  - Liu C
AD  - Department of Biology, Pennsylvania State University, University Park, 
      Pennsylvania 16802, USA.
FAU - Axtell, Michael J
AU  - Axtell MJ
FAU - Fedoroff, Nina V
AU  - Fedoroff NV
LA  - eng
SI  - GEO/GSE29802
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20120403
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (HYL1 protein, Arabidopsis)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 3.1.26.3 (DCL1 protein, Arabidopsis)
RN  - EC 3.1.26.3 (Ribonuclease III)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Alleles
MH  - Amino Acid Sequence
MH  - Arabidopsis/*enzymology/genetics
MH  - Arabidopsis Proteins/genetics/*metabolism
MH  - Base Sequence
MH  - *Catalytic Domain
MH  - Cell Cycle Proteins/genetics/*metabolism
MH  - Cloning, Molecular
MH  - Enzyme Activation
MH  - Genetic Complementation Test
MH  - Genetic Pleiotropy
MH  - MicroRNAs/genetics/*metabolism
MH  - Molecular Sequence Data
MH  - Phenotype
MH  - Plants, Genetically Modified/enzymology/genetics
MH  - Protein Structure, Tertiary
MH  - RNA-Binding Proteins/genetics/metabolism
MH  - Recombinant Proteins/genetics/metabolism
MH  - Ribonuclease III/genetics/*metabolism
PMC - PMC3406889
EDAT- 2012/04/05 06:00
MHDA- 2012/10/10 06:00
PMCR- 2012/04/03
CRDT- 2012/04/05 06:00
PHST- 2012/04/05 06:00 [entrez]
PHST- 2012/04/05 06:00 [pubmed]
PHST- 2012/10/10 06:00 [medline]
PHST- 2012/04/03 00:00 [pmc-release]
AID - pp.112.193508 [pii]
AID - 193508 [pii]
AID - 10.1104/pp.112.193508 [doi]
PST - ppublish
SO  - Plant Physiol. 2012 Jun;159(2):748-58. doi: 10.1104/pp.112.193508. Epub 2012 Apr 
      3.