PMID- 22474985
OWN - NLM
STAT- MEDLINE
DCOM- 20130124
LR  - 20120405
IS  - 1009-3591 (Print)
IS  - 1009-3591 (Linking)
VI  - 18
IP  - 3
DP  - 2012 Mar
TI  - [Protective effect of ERbeta on penile vascular endothelium in mice].
PG  - 216-21
AB  - OBJECTIVE: To investigate the protective effect of ERbeta on the penile vascular 
      endothelium in mice. METHODS: We randomly selected 12 ERbeta knockout (ERbetaKO) 
      and 12 C57BL/6 male mice, and divided them into four groups: normal control, 
      ERbetaKO, ERbetaKO + TNFalpha, and wild-type + TNFalpha group. The former two 
      were treated with normal saline, while the latter two by intraperitoneal 
      injection of TNFalpha at 6 microg/(kg x d) for 14 days. Then we observed the 
      spontaneous erectile response induced by APO and changes of the endothelial cells 
      by immunohistochemical staining of CD34 and vWF, and detected cell apoptosis in 
      the penile cavernous tissue by TUNEL. RESULTS: Compared with the normal control 
      group, the ERbetaKO group showed significantly increased erectile latency 
      (P<0.05), but no significant difference in the number of erections; the ERbetaKO 
      + TNFalpha and wild-type + TNFalpha groups, too, exhibited remarkably longer 
      erectile latency (P<0.05) but fewer erections (P<0.05), with even more obvious 
      changes in the ERbetaKO + TNFalpha group. The expressions of CD34 and vWF were 
      significantly reduced in the ERbetaKO group (2.25 +/- 0.50 and 2.00 +/- 0.00), 
      ERbetaKO + TNFalpha group (0.25 +/- 0.50 and 0.33 +/- 0.58) and wild-type + 
      TNFalpha group (1.50 +/- 0.58 and 1.25 +/- 0.50) as compared with those in the 
      control (3.00 +/- 0.00 and 2.75 +/- 0.50) (P<0.05), even lower in the ERbetaKO + 
      TNFalpha than in the wild-type + TNFalpha group (P<0.05). Apoptotic cells were 
      found only in the ERbetaKO + TNFalpha group. CONCLUSION: After ERbeta knockout 
      and especially after treated with the endothelial injury factor TNFalpha, 
      endothelial cells are decreased in the penile vessels in mice, which suggests 
      that ERbeta has a protective effect on the penile cavernous sinus endothelium.
FAU - Ma, Jie-Hua
AU  - Ma JH
AD  - College of Life Science, Nanjing Normal University, Nanjing, Jiangsu 210046, 
      China.
FAU - Shi, Hou-Xia
AU  - Shi HX
FAU - Pan, Lian-Jun
AU  - Pan LJ
FAU - Huang, Yu-Feng
AU  - Huang YF
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Nan Ke Xue
JT  - Zhonghua nan ke xue = National journal of andrology
JID - 101093592
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Endothelium, Vascular/cytology/*drug effects
MH  - Estrogen Receptor beta/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Penile Erection/drug effects
MH  - Penis/*blood supply/drug effects
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2012/04/06 06:00
MHDA- 2013/01/25 06:00
CRDT- 2012/04/06 06:00
PHST- 2012/04/06 06:00 [entrez]
PHST- 2012/04/06 06:00 [pubmed]
PHST- 2013/01/25 06:00 [medline]
PST - ppublish
SO  - Zhonghua Nan Ke Xue. 2012 Mar;18(3):216-21.