PMID- 22475781
OWN - NLM
STAT- MEDLINE
DCOM- 20120829
LR  - 20131121
IS  - 1873-5487 (Electronic)
IS  - 0188-4409 (Linking)
VI  - 43
IP  - 2
DP  - 2012 Feb
TI  - Effectiveness of silver-enhanced in situ hybridization for evaluating HER2 gene 
      status in invasive breast carcinoma: a comparative study.
PG  - 139-44
LID - 10.1016/j.arcmed.2012.03.010 [doi]
AB  - BACKGROUND AND AIMS: HER2 gene amplification occurs in breast cancers and has 
      implications for treatment and prognosis. Recently, a new direct evaluation 
      technique, silver enhanced in situ hybridization (SISH) was developed for 
      evaluating HER2 gene status. This study was performed to evaluate the SISH 
      technique for clinical use by comparing it to that of fluorescence in situ 
      hybridization (FISH) and immunohistochemistry (IHC). METHODS: We studied 543 
      cases of excised breast specimens diagnosed as invasive ductal carcinoma by IHC, 
      FISH, and SISH using a tissue microarray. IHC, FISH, and SISH results were 
      interpreted according to the American Society of Clinical Oncology/College of 
      American Pathologists guidelines. A total of seven English studies that reported 
      the concordance rates of SISH and BDISH compared to FISH published before July 
      2011 were retrieved. RESULTS: The consensus concordance rate between SISH and 
      FISH was 96.69% (kappa value = 0.9175). The pooled sensitivity was 0.94 [95% 
      confidence interval (CI) = 0.91-0.97], and the pooled specificity was 0.98 (95% 
      CI = 0.96-099) in a meta-analysis of the retrieved studies and this study. Area 
      under the receiver operating characteristics curve was 0.9906. CONCLUSIONS: SISH 
      technique is an effective modality and is comparable with FISH for evaluating 
      HER2 gene amplification in patients with breast carcinoma.
CI  - Copyright (c) 2012 IMSS. All rights reserved.
FAU - Lee, Youngseok
AU  - Lee Y
AD  - Department of Pathology, Korea University Guro Hospital, Guro-gu, Seoul, Korea.
FAU - Ryu, Youngjoon
AU  - Ryu Y
FAU - Jeong, Hoiseon
AU  - Jeong H
FAU - Chang, Hyeyoon
AU  - Chang H
FAU - Kim, Younghye
AU  - Kim Y
FAU - Kim, Aeree
AU  - Kim A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20120401
PL  - United States
TA  - Arch Med Res
JT  - Archives of medical research
JID - 9312706
RN  - 3M4G523W1G (Silver)
SB  - IM
MH  - Breast Neoplasms/*genetics/pathology
MH  - *Genes, erbB-2
MH  - Humans
MH  - In Situ Hybridization/*methods
MH  - Neoplasm Invasiveness
MH  - Silver/*chemistry
EDAT- 2012/04/06 06:00
MHDA- 2012/08/30 06:00
CRDT- 2012/04/06 06:00
PHST- 2012/01/02 00:00 [received]
PHST- 2012/03/07 00:00 [accepted]
PHST- 2012/04/06 06:00 [entrez]
PHST- 2012/04/06 06:00 [pubmed]
PHST- 2012/08/30 06:00 [medline]
AID - S0188-4409(12)00080-X [pii]
AID - 10.1016/j.arcmed.2012.03.010 [doi]
PST - ppublish
SO  - Arch Med Res. 2012 Feb;43(2):139-44. doi: 10.1016/j.arcmed.2012.03.010. Epub 2012 
      Apr 1.