PMID- 22477041
OWN - NLM
STAT- MEDLINE
DCOM- 20131203
LR  - 20211021
IS  - 0973-7693 (Electronic)
IS  - 0019-5456 (Linking)
VI  - 79
IP  - 12
DP  - 2012 Dec
TI  - Prenatal diagnosis of LAD-I on cord blood by flowcytometry.
PG  - 1605-9
LID - 10.1007/s12098-012-0737-5 [doi]
AB  - OBJECTIVES: To optimize a simple flowcytometric technique for Prenatal diagnosis 
      (PND) for Leukocyte adhesions defect (LAD-I) on cordocentesis sample at 18 wk 
      gestation. METHODS: Normal reference ranges for expression of CD18/CD11-integrins 
      in neutrophils and lymphocytes at 18 wk of gestation were established by 
      flowcytometry. PND for LAD-I was then performed on the cordocentesis samples in 
      three 'at risk' pregnancies after ruling out maternal contamination. RESULTS: 
      CD18 and CD11a expression on fetal lymphocytes were found to be the most useful 
      parameters for PND of LAD-I. All the three fetuses tested showed normal 
      expression of CD18/CD11-integrins and thus were unaffected. This was confirmed by 
      testing the cord blood (CB) samples after delivery and normal growth and absence 
      of serious infections on follow-up. CONCLUSIONS: Flowcytometry offers a rapid and 
      sensitive technique for PND of LAD-I in the absence of facilities for molecular 
      diagnosis. Obstetricians, even in developing countries with modest facilities, 
      can offer considerable relief for the families.
FAU - Madkaikar, Manisha Rajan
AU  - Madkaikar MR
AD  - National Institute of Immunohematlogy, Pediatric Immunology and Leukocyte 
      Biology, 13th Flr, KEM Hospital, Parel, Mumbai, Maharashtra, 400012, India. 
      madkaikarm@icmr.org.in
FAU - Gupta, Maya
AU  - Gupta M
FAU - Rao, Meghana
AU  - Rao M
FAU - Ghosh, Kanjaksha
AU  - Ghosh K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120404
PL  - India
TA  - Indian J Pediatr
JT  - Indian journal of pediatrics
JID - 0417442
RN  - 0 (CD11 Antigens)
RN  - 0 (CD18 Antigens)
SB  - IM
MH  - Adult
MH  - CD11 Antigens/blood
MH  - CD18 Antigens/blood
MH  - Female
MH  - *Fetal Blood
MH  - Flow Cytometry
MH  - Gestational Age
MH  - Humans
MH  - Leukocyte-Adhesion Deficiency Syndrome/*blood/*diagnosis
MH  - Pregnancy
MH  - *Prenatal Diagnosis
EDAT- 2012/04/06 06:00
MHDA- 2013/12/16 06:00
CRDT- 2012/04/06 06:00
PHST- 2011/07/26 00:00 [received]
PHST- 2012/03/14 00:00 [accepted]
PHST- 2012/04/06 06:00 [entrez]
PHST- 2012/04/06 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
AID - 10.1007/s12098-012-0737-5 [doi]
PST - ppublish
SO  - Indian J Pediatr. 2012 Dec;79(12):1605-9. doi: 10.1007/s12098-012-0737-5. Epub 
      2012 Apr 4.