PMID- 22479673
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20120823
LR  - 20240315
IS  - 2041-2851 (Electronic)
VI  - 2012
DP  - 2012
TI  - Cytogenetic characterization and genome size of the medicinal plant Catharanthus 
      roseus (L.) G. Don.
PG  - pls002
LID - 10.1093/aobpla/pls002 [doi]
LID - pls002
AB  - BACKGROUND AND AIMS: Catharanthus roseus is a highly valuable medicinal plant 
      producing several terpenoid indole alkaloids (TIAs) with pharmaceutical 
      applications, including the anticancer agents vinblastine and vincristine. Due to 
      the interest in its TIAs, C. roseus is one of the most extensively studied 
      medicinal plants and has become a model species for the study of plant secondary 
      metabolism. However, very little is known about the cytogenetics and genome size 
      of this species, in spite of their importance for breeding programmes, TIA 
      genetics and emerging genomic research. Therefore, the present paper provides a 
      karyotype description and fluorescence in situ hybridization (FISH) data for C. 
      roseus, as well as a rigorous characterization of its genome size. METHODOLOGY: 
      The organization of C. roseus chromosomes was characterized using several 
      DNA/chromatin staining techniques and FISH of rDNA. Genome size was investigated 
      by flow cytometry using an optimized methodology. PRINCIPAL RESULTS: The C. 
      roseus full chromosome complement of 2n = 16 includes two metacentric, four 
      subtelocentric and two telocentric chromosome pairs, with the presence of a 
      single nucleolus organizer region in chromosome 6. An easy and reliable flow 
      cytometry protocol for nuclear genome analysis of C. roseus was optimized, and 
      the C-value of this species was estimated to be 1C = 0.76 pg, corresponding to 
      738 Mbp. CONCLUSIONS: The organization and size of the C. roseus genome were 
      characterized, providing an important basis for future studies of this important 
      medicinal species, including further cytogenetic mapping, genomics, TIA genetics 
      and breeding programmes.
FAU - Guimaraes, Guilherme
AU  - Guimaraes G
AD  - IBMC - Instituto de Biologia Molecular e Celular , Universidade do Porto , Rua do 
      Campo Alegre, 823, 4150-180 Porto , Portugal.
FAU - Cardoso, Luisa
AU  - Cardoso L
FAU - Oliveira, Helena
AU  - Oliveira H
FAU - Santos, Conceicao
AU  - Santos C
FAU - Duarte, Patricia
AU  - Duarte P
FAU - Sottomayor, Mariana
AU  - Sottomayor M
LA  - eng
PT  - Journal Article
DEP - 20120302
PL  - England
TA  - AoB Plants
JT  - AoB PLANTS
JID - 101539425
PMC - PMC3292738
EDAT- 2012/04/06 06:00
MHDA- 2012/04/06 06:01
PMCR- 2012/01/01
CRDT- 2012/04/06 06:00
PHST- 2011/09/08 00:00 [received]
PHST- 2012/01/05 00:00 [accepted]
PHST- 2012/04/06 06:00 [entrez]
PHST- 2012/04/06 06:00 [pubmed]
PHST- 2012/04/06 06:01 [medline]
PHST- 2012/01/01 00:00 [pmc-release]
AID - pls002 [pii]
AID - 10.1093/aobpla/pls002 [doi]
PST - ppublish
SO  - AoB Plants. 2012;2012:pls002. doi: 10.1093/aobpla/pls002. Epub 2012 Mar 2.