PMID- 22480259
OWN - NLM
STAT- MEDLINE
DCOM- 20121220
LR  - 20181201
IS  - 1543-8392 (Electronic)
IS  - 1543-8384 (Linking)
VI  - 9
IP  - 5
DP  - 2012 May 7
TI  - Dendrimer-driven neurotrophin expression differs in temporal patterns between 
      rodent and human stem cells.
PG  - 1521-8
LID - 10.1021/mp300041k [doi]
AB  - This study reports the use of a nonviral expression system based on 
      polyamidoamine dendrimers for time-restricted neurotrophin overproduction in 
      mesenchymal stem cells and skin precursor-derived Schwann cells. The dendrimers 
      were used to deliver plasmids for brain-derived neurotrophic factor (BDNF) or 
      neurotrophin-3 (NT-3) expression in both rodent and human stem cells, and the 
      timelines of expression were studied. We have found that, despite the fact that 
      transfection efficiencies and protein expression levels were comparable, 
      dendrimer-driven expression in human mesenchymal stem cells was characterized by 
      a more rapid decline compared to rodent cells. Transient expression systems can 
      be beneficial for some neurotrophins, which were earlier reported to cause 
      unwanted side effects in virus-based long-term expression models. Nonviral 
      neurotrophin expression is a biologically safe and accessible alternative to 
      increase the therapeutic potential of autologous adult stem cells and stem 
      cell-derived functional differentiated cells.
FAU - Shakhbazau, Antos
AU  - Shakhbazau A
AD  - Department of Clinical Neurosciences, Faculty of Medicine, University of Calgary, 
      Calgary, Canada. shakhbazau@gmail.com
FAU - Shcharbin, Dzmitry
AU  - Shcharbin D
FAU - Seviaryn, Ihar
AU  - Seviaryn I
FAU - Goncharova, Natalya
AU  - Goncharova N
FAU - Kosmacheva, Svetlana
AU  - Kosmacheva S
FAU - Potapnev, Mihail
AU  - Potapnev M
FAU - Bryszewska, Maria
AU  - Bryszewska M
FAU - Kumar, Ranjan
AU  - Kumar R
FAU - Biernaskie, Jeffrey
AU  - Biernaskie J
FAU - Midha, Rajiv
AU  - Midha R
LA  - eng
GR  - 163322/Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120419
PL  - United States
TA  - Mol Pharm
JT  - Molecular pharmaceutics
JID - 101197791
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Dendrimers)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Cell Differentiation/genetics/physiology
MH  - Cells, Cultured
MH  - Chondrogenesis/genetics/physiology
MH  - Dendrimers/*chemistry
MH  - Humans
MH  - Mesenchymal Stem Cells/cytology/metabolism
MH  - Nerve Growth Factors/genetics/*metabolism
MH  - Neurotrophin 3/genetics/metabolism
MH  - Osteogenesis/genetics/physiology
MH  - Plasmids/genetics
MH  - Transfection/methods
EDAT- 2012/04/07 06:00
MHDA- 2012/12/21 06:00
CRDT- 2012/04/07 06:00
PHST- 2012/04/07 06:00 [entrez]
PHST- 2012/04/07 06:00 [pubmed]
PHST- 2012/12/21 06:00 [medline]
AID - 10.1021/mp300041k [doi]
PST - ppublish
SO  - Mol Pharm. 2012 May 7;9(5):1521-8. doi: 10.1021/mp300041k. Epub 2012 Apr 19.