PMID- 22480947
OWN - NLM
STAT- MEDLINE
DCOM- 20121001
LR  - 20220330
IS  - 1878-7568 (Electronic)
IS  - 1742-7061 (Linking)
VI  - 8
IP  - 7
DP  - 2012 Jul
TI  - Enhancement of bone regeneration through facile surface functionalization of 
      solid freeform fabrication-based three-dimensional scaffolds using mussel 
      adhesive proteins.
PG  - 2578-86
LID - 10.1016/j.actbio.2012.03.041 [doi]
AB  - Solid freeform fabrication (SFF) is recognized as a promising tool for creating 
      tissue engineering scaffolds due to advantages such as superior interconnectivity 
      and highly porous structure. Despite structural support for SFF-based 
      three-dimensional (3-D) scaffolds that can lead to tissue regeneration, lack of 
      cell recognition motifs and/or biochemical factors has been considered a 
      limitation. Previously, recombinant mussel adhesive proteins (MAPs) were 
      successfully demonstrated to be functional cell adhesion materials on various 
      surfaces due to their peculiar adhesive properties. Herein, MAPs were applied as 
      surface functionalization materials to SFF-based 3-D 
      polycaprolactone/poly(lactic-co-glycolic acid) scaffolds. We successfully coated 
      MAPs onto scaffold surfaces by simply dipping the scaffolds into the MAP 
      solution, which was confirmed through X-ray photoelectron spectroscopy and 
      scanning electron microscopy analyses. Through in vitro study using human adipose 
      tissue-derived stem cells (hADSCs), significant enhancement of cellular 
      activities such as attachment, proliferation, and osteogenic differentiation was 
      observed on MAP-coated 3-D scaffolds, especially on which fused 
      arginine-glycine-aspartic acid peptides were efficiently exposed. In addition, we 
      found that in vivo hADSC implantation with MAP-coated scaffolds enhanced bone 
      regeneration in a rat calvarial defect model. These results collectively 
      demonstrate that facile surface functionalization of 3-D scaffolds using MAP 
      would be a promising strategy for successful tissue engineering applications.
CI  - Copyright (c) 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights 
      reserved.
FAU - Hong, Jung Min
AU  - Hong JM
AD  - Department of Mechanical Engineering, Pohang University of Science and 
      Technology, Republic of Korea.
FAU - Kim, Bum Jin
AU  - Kim BJ
FAU - Shim, Jin-Hyung
AU  - Shim JH
FAU - Kang, Kyung Shin
AU  - Kang KS
FAU - Kim, Ki-Joo
AU  - Kim KJ
FAU - Rhie, Jong Won
AU  - Rhie JW
FAU - Cha, Hyung Joon
AU  - Cha HJ
FAU - Cho, Dong-Woo
AU  - Cho DW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120402
PL  - England
TA  - Acta Biomater
JT  - Acta biomaterialia
JID - 101233144
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (Oligopeptides)
RN  - 0 (Polyesters)
RN  - 0 (Proteins)
RN  - 0 (adhesive protein, mussel)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 24980-41-4 (polycaprolactone)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 78VO7F77PN (arginyl-glycyl-aspartic acid)
SB  - IM
MH  - Adipose Tissue/cytology
MH  - Animals
MH  - Bone Regeneration/*drug effects
MH  - Cell Adhesion/drug effects
MH  - Cell Differentiation/drug effects
MH  - Cell Proliferation/drug effects
MH  - Coated Materials, Biocompatible/pharmacology
MH  - Humans
MH  - Lactic Acid/pharmacology
MH  - Male
MH  - Oligopeptides/pharmacology
MH  - Osteogenesis/drug effects
MH  - Photoelectron Spectroscopy
MH  - Polyesters/pharmacology
MH  - Polyglycolic Acid/pharmacology
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Proteins/*pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Skull/diagnostic imaging/drug effects/pathology
MH  - Stem Cells/cytology/drug effects/metabolism
MH  - Surface Properties/drug effects
MH  - Tissue Engineering/*methods
MH  - Tissue Scaffolds/*chemistry
MH  - X-Ray Microtomography
EDAT- 2012/04/07 06:00
MHDA- 2012/10/02 06:00
CRDT- 2012/04/07 06:00
PHST- 2011/12/22 00:00 [received]
PHST- 2012/03/03 00:00 [revised]
PHST- 2012/03/27 00:00 [accepted]
PHST- 2012/04/07 06:00 [entrez]
PHST- 2012/04/07 06:00 [pubmed]
PHST- 2012/10/02 06:00 [medline]
AID - S1742-7061(12)00136-5 [pii]
AID - 10.1016/j.actbio.2012.03.041 [doi]
PST - ppublish
SO  - Acta Biomater. 2012 Jul;8(7):2578-86. doi: 10.1016/j.actbio.2012.03.041. Epub 
      2012 Apr 2.