PMID- 22484065
OWN - NLM
STAT- MEDLINE
DCOM- 20120907
LR  - 20120508
IS  - 1873-4367 (Electronic)
IS  - 0927-7765 (Linking)
VI  - 95
DP  - 2012 Jun 15
TI  - Elaboration and characterization of nanoliposome made of soya; rapeseed and 
      salmon lecithins: application to cell culture.
PG  - 75-81
LID - 10.1016/j.colsurfb.2012.02.024 [doi]
AB  - Health benefits of unsaturated fatty acids have been demonstrated over the last 
      decades. Nanotechnology provided new process to produce particles such as 
      liposomes and nanoliposomes made of pure phospholipids. These techniques are 
      already used in pharmaceutics to augment the bioavailability and the 
      bioefficiency of drugs. The aim of this paper is to characterize and evaluate the 
      potential of nanoliposomes made of three lecithins (soya, rapeseed and salmon) on 
      cell culture in order to use them in the future as drug delivery systems for 
      tissue engineering. We began to measure, with zetasizer, the radius size of 
      liposomes particles which are 125.5, 136.7 and 130.3 nm respectively for 
      rapeseed, soya and salmon lecithin. Simultaneously, solutions observed by TEM 
      demonstrated the particles were made much of liposomes than droplet (emulsion). 
      Finally, we found that the solutions of lecithins were enough stable over 5 days 
      at 37  degrees C to be used in culture medium. We investigated the effect of soya, 
      rapeseed and salmon lecithin liposome from 2mg/mL to 5.2 mug/mL on metabolic 
      activity and cell proliferation on rat bone marrow stem cells (rBMSC) during 14 
      days. The results showed that the three lecithins (soya, rapeseed and salmon) 
      improve cell proliferation at different concentration.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Arab Tehrany, Elmira
AU  - Arab Tehrany E
AD  - Laboratoire d'Ingenierie des Biomolecules, Nancy-Universite, 2 avenue de Foret de 
      Haye, 54505 Vandoeuvre-Les-Nancy, France. 
      elmira.arab-tehrany@ensaia.inpl-nancy.fr
FAU - Kahn, Cyril J F
AU  - Kahn CJ
FAU - Baravian, Christophe
AU  - Baravian C
FAU - Maherani, Behnoush
AU  - Maherani B
FAU - Belhaj, Nabila
AU  - Belhaj N
FAU - Wang, Xiong
AU  - Wang X
FAU - Linder, Michel
AU  - Linder M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120310
PL  - Netherlands
TA  - Colloids Surf B Biointerfaces
JT  - Colloids and surfaces. B, Biointerfaces
JID - 9315133
RN  - 0 (Lecithins)
RN  - 0 (Liposomes)
RN  - 8001-22-7 (Soybean Oil)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology/*drug effects
MH  - Brassica rapa/*chemistry
MH  - Cell Culture Techniques
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Drug
MH  - Drug Delivery Systems
MH  - Lecithins/chemistry/*pharmacology
MH  - Liposomes/chemistry/*pharmacology
MH  - Male
MH  - Nanoparticles/*chemistry
MH  - Particle Size
MH  - Rats
MH  - Rats, Wistar
MH  - *Salmon
MH  - Soybean Oil/chemistry/*pharmacology
MH  - Structure-Activity Relationship
MH  - Surface Properties
EDAT- 2012/04/10 06:00
MHDA- 2012/09/08 06:00
CRDT- 2012/04/10 06:00
PHST- 2011/11/06 00:00 [received]
PHST- 2012/02/10 00:00 [revised]
PHST- 2012/02/10 00:00 [accepted]
PHST- 2012/04/10 06:00 [entrez]
PHST- 2012/04/10 06:00 [pubmed]
PHST- 2012/09/08 06:00 [medline]
AID - S0927-7765(12)00135-X [pii]
AID - 10.1016/j.colsurfb.2012.02.024 [doi]
PST - ppublish
SO  - Colloids Surf B Biointerfaces. 2012 Jun 15;95:75-81. doi: 
      10.1016/j.colsurfb.2012.02.024. Epub 2012 Mar 10.