PMID- 22484241
OWN - NLM
STAT- MEDLINE
DCOM- 20120827
LR  - 20120430
IS  - 1096-0023 (Electronic)
IS  - 1043-4666 (Linking)
VI  - 58
IP  - 3
DP  - 2012 Jun
TI  - The anti-inflammatory actions of IL-4 in human monocytes are not mediated by 
      IL-10, RP105 or the kinase activity of RIPK2.
PG  - 415-23
LID - 10.1016/j.cyto.2012.03.009 [doi]
AB  - The anti-inflammatory actions of IL-4 in activated human monocytes may reflect 
      transcriptional regulation of genes involved in TLR signaling pathways. Tailored 
      gene arrays were conducted to profile the expression of 84 genes central to 
      TLR-mediated signal transduction in human monocytes treated with the TLR4 ligand, 
      LPS, with or without IL-4. In the first 3h, IL-4 down-regulated mRNA levels of 
      LPS-induced inflammatory cytokines and chemokines, without altering mRNA levels 
      of TLRs, TLR-related signaling molecules or multiple transcription factors. The 
      down-regulation of inflammatory genes by IL-4 was preceded by an early 
      up-regulation of IL-10 mRNA and protein and mRNA for receptor-interacting 
      serine-threonine kinase 2 (RIPK2), the TLR homolog, RP105, and c-Maf, a 
      transcription factor required for IL-10 gene expression. However, IL-4 still 
      suppressed LPS-induced TNFalpha production in bone-marrow derived macrophages from 
      IL10(-/-) mice, and in the presence of a neutralizing antibody to IL-10 in human 
      monocytes. The up-regulation of RIPK2 and RP105 mRNA by IL-4 occurred 
      independently of IL-10. IL-4 maintained the ability to suppress LPS-induced TNFalpha 
      and enhance IL-10 production in the presence of RIPK2 kinase inhibitors. Further, 
      IL-4 failed to up-regulate expression of RP105 at the cell surface. In 
      conclusion, the anti-inflammatory actions of IL-4 occur independently of IL-10, 
      RP105, and the kinase activity of RIPK2.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Woodward, Eleanor A
AU  - Woodward EA
AD  - Inflammation Laboratory, Telethon Institute for Child Health Research and Centre 
      for Child Health Research, University of Western Australia, GPO Box 855, West 
      Perth 6872, Australia.
FAU - Kolesnik, Tatiana B
AU  - Kolesnik TB
FAU - Nicholson, Sandra E
AU  - Nicholson SE
FAU - Prele, Cecilia M
AU  - Prele CM
FAU - Hart, Prue H
AU  - Hart PH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120407
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Antigens, CD)
RN  - 0 (CD180 protein, human)
RN  - 0 (DNA Primers)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
RN  - EC 2.7.11.1 (RIPK2 protein, human)
RN  - EC 2.7.11.1 (Receptor-Interacting Protein Serine-Threonine Kinase 2)
SB  - IM
MH  - Animals
MH  - Antigens, CD/*physiology
MH  - Base Sequence
MH  - DNA Primers
MH  - Flow Cytometry
MH  - Humans
MH  - Inflammation/*prevention & control
MH  - Interleukin-10/genetics/*physiology
MH  - Interleukin-4/*physiology
MH  - Mice
MH  - Monocytes/*physiology
MH  - Real-Time Polymerase Chain Reaction
MH  - Receptor-Interacting Protein Serine-Threonine Kinase 2/*physiology
EDAT- 2012/04/10 06:00
MHDA- 2012/08/28 06:00
CRDT- 2012/04/10 06:00
PHST- 2011/12/06 00:00 [received]
PHST- 2012/02/23 00:00 [revised]
PHST- 2012/03/12 00:00 [accepted]
PHST- 2012/04/10 06:00 [entrez]
PHST- 2012/04/10 06:00 [pubmed]
PHST- 2012/08/28 06:00 [medline]
AID - S1043-4666(12)00092-0 [pii]
AID - 10.1016/j.cyto.2012.03.009 [doi]
PST - ppublish
SO  - Cytokine. 2012 Jun;58(3):415-23. doi: 10.1016/j.cyto.2012.03.009. Epub 2012 Apr 
      7.