PMID- 22484926
OWN - NLM
STAT- MEDLINE
DCOM- 20121119
LR  - 20181201
IS  - 1347-6947 (Electronic)
IS  - 0916-8451 (Linking)
VI  - 76
IP  - 4
DP  - 2012
TI  - SHRSP/Izm and WKY/NCrlCrlj rats having a missense mutation in Abcg5 deposited 
      plant sterols in the body, but did not change their biliary secretion and 
      lymphatic absorption-comparison with Jcl:Wistar and WKY/Izm rats.
PG  - 660-4
AB  - We had previously found plant sterols deposited in the bodies of stroke-prone 
      spontaneously hypertensive rats (SHRSP)/Sea and Wistar Kyoto (WKY)/NCrlCrlj rats 
      that had a missense mutation in the Abcg5 cDNA sequence that coded for 
      ATP-binding cassette transporter (ABC) G5. We used SHRSP/Izm, WKY/NCrlCrlj, and 
      WKY/Izm rats in the present study to determine the mechanisms for plant sterol 
      deposition in the body. Jcl:Wistar rats were used as a control strain. A diet 
      containing 0.5% plant sterols fed to the rats resulted in plant sterol deposition 
      in the body of SHRSP/Izm, but not in WKY/Izm or Jcl:Wistar rats. Only a single 
      non-synonymous nucleotide change, G1747T, resulting in a conservative cysteine 
      substitution for glycine at amino acid 583 (Gly583Cys) in Abcg5 cDNA was 
      identified in the SHRSP/Izm and WKY/NCrlCrlj rats. However, this mutation was not 
      found in the WKY/Izm or Jcl:Wistar rats. No significant difference in the biliary 
      secretion or lymphatic absorption of plant sterols was apparent between the rat 
      strains with or without the missense mutation in Abcg5 cDNA. Our observations 
      suggest that plant sterol deposition in rat strains with the missense mutation in 
      Abcg5 cDNA can occur, despite there being no significant change in the biliary 
      secretion or lymphatic absorption of plant sterols.
FAU - Kato, Masaki
AU  - Kato M
AD  - Laboratory of Food and Biomolecular Science, Graduate School of Agricultural 
      Science, Tohoku University, Sendai, Japan.
FAU - Ito, Yusuke
AU  - Ito Y
FAU - Tanaka, Yasutake
AU  - Tanaka Y
FAU - Sato, Masao
AU  - Sato M
FAU - Imaizumi, Katsumi
AU  - Imaizumi K
FAU - Inoue, Nao
AU  - Inoue N
FAU - Ikeda, Ikuo
AU  - Ikeda I
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20120407
PL  - England
TA  - Biosci Biotechnol Biochem
JT  - Bioscience, biotechnology, and biochemistry
JID - 9205717
RN  - 0 (ATP Binding Cassette Transporter, Subfamily G, Member 5)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Abcg5 protein, rat)
RN  - 0 (Lipoproteins)
RN  - 0 (Nucleotides)
RN  - 0 (Phytosterols)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily G, Member 5
MH  - ATP-Binding Cassette Transporters/*genetics/metabolism
MH  - Absorption
MH  - Amino Acid Substitution
MH  - Animals
MH  - Bile/*metabolism
MH  - Blood Pressure
MH  - Hypertension/*genetics/metabolism/physiopathology
MH  - Lipoproteins/*genetics/metabolism
MH  - Lymphatic Vessels/*metabolism/physiopathology
MH  - Male
MH  - Mutation, Missense/*genetics
MH  - Nucleotides
MH  - Phytosterols/administration & dosage/metabolism
MH  - Rats
MH  - Rats, Inbred SHR/genetics/metabolism
MH  - Rats, Inbred WKY/genetics/metabolism
MH  - Rats, Wistar/genetics/metabolism
EDAT- 2012/04/10 06:00
MHDA- 2012/12/10 06:00
CRDT- 2012/04/10 06:00
PHST- 2012/04/10 06:00 [entrez]
PHST- 2012/04/10 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
AID - JST.JSTAGE/bbb/110667 [pii]
AID - 10.1271/bbb.110667 [doi]
PST - ppublish
SO  - Biosci Biotechnol Biochem. 2012;76(4):660-4. doi: 10.1271/bbb.110667. Epub 2012 
      Apr 7.