PMID- 22488873
OWN - NLM
STAT- MEDLINE
DCOM- 20121101
LR  - 20161125
IS  - 1439-7633 (Electronic)
IS  - 1439-4227 (Linking)
VI  - 13
IP  - 7
DP  - 2012 May 7
TI  - Characterization of volatile organic compounds in human leukocyte antigen 
      heterologous expression systems: a cell's "chemical odor fingerprint".
PG  - 1053-9
LID - 10.1002/cbic.201200011 [doi]
AB  - The major histocompatibility complex (MHC), or human leukocyte antigen (HLA) 
      gene-coding region in humans, plays a significant role in infectious disease 
      response, autoimmunity, and cellular recognition. This super locus is essential 
      in mate selection and kin recognition because of the organism-specific odor which 
      can be perceived by other individuals. However, how the unique MHC genetic 
      combination of an organism correlates with generation of the organism-specific 
      odor is not well understood. In the present work, we have shown that human 
      B-cells produce a set of volatile organic compounds (VOCs) that can be measured 
      by GC-MS. More importantly, our results show that specific HLA alleles are 
      related to production of selected VOCs, and that this leads to a cell-specific 
      odor "fingerprint". We used a C1R HLA class I A and B locus negative cell line, 
      along with C1R cell lines that were stably transfected with specific A and B 
      alleles. Our work demonstrates for the first time that HLA alleles can directly 
      influence production of specific odor compounds at the cellular level. Given that 
      the resulting odor fingerprint depends on expression of specific HLA sequences, 
      it may yield information on unique human scent profiles, composition of exhaled 
      breath, as well as immune response states in future studies.
CI  - Copyright (c) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Aksenov, Alexander A
AU  - Aksenov AA
AD  - Department of Mechanical and Aerospace Engineering, University of California, 
      Davis, CA 95616, USA.
FAU - Gojova, Andrea
AU  - Gojova A
FAU - Zhao, Weixiang
AU  - Zhao W
FAU - Morgan, Joshua T
AU  - Morgan JT
FAU - Sankaran, Shankar
AU  - Sankaran S
FAU - Sandrock, Christian E
AU  - Sandrock CE
FAU - Davis, Cristina E
AU  - Davis CE
LA  - eng
GR  - UL1 RR024 146/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20120404
PL  - Germany
TA  - Chembiochem
JT  - Chembiochem : a European journal of chemical biology
JID - 100937360
RN  - 0 (HLA Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Volatile Organic Compounds)
SB  - IM
MH  - Breath Tests/methods
MH  - Cell Line
MH  - Gas Chromatography-Mass Spectrometry
MH  - HLA Antigens/*chemistry/genetics/immunology
MH  - Histocompatibility Antigens Class I/*chemistry/genetics/immunology
MH  - Humans
MH  - Odorants
MH  - Transfection
MH  - Volatile Organic Compounds/*chemistry/metabolism
EDAT- 2012/04/11 06:00
MHDA- 2012/11/02 06:00
CRDT- 2012/04/11 06:00
PHST- 2012/01/06 00:00 [received]
PHST- 2012/04/11 06:00 [entrez]
PHST- 2012/04/11 06:00 [pubmed]
PHST- 2012/11/02 06:00 [medline]
AID - 10.1002/cbic.201200011 [doi]
PST - ppublish
SO  - Chembiochem. 2012 May 7;13(7):1053-9. doi: 10.1002/cbic.201200011. Epub 2012 Apr 
      4.