PMID- 22489537
OWN - NLM
STAT- MEDLINE
DCOM- 20120723
LR  - 20211021
IS  - 1365-2141 (Electronic)
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 157
IP  - 6
DP  - 2012 Jun
TI  - Search for an aetiological virus candidate in chronic lymphocytic leukaemia by 
      extensive transcriptome analysis.
PG  - 709-17
LID - 10.1111/j.1365-2141.2012.09116.x [doi]
AB  - As an approach to determining the aetiology of chronic lymphocytic leukaemia 
      (CLL), we searched for a virus expressed in human CLL B-cells by combining 
      high-throughput sequencing and digital subtraction. Pooled B-cell mRNA 
      transcriptomes from five CLL patients and five healthy donors were sequenced with 
      454 Life Sciences technology. Human reads were excluded by BLAST (Basic Local 
      Alignment Search Tool) and BLAT (BLAST-like alignment tool) searches. Remaining 
      reads were screened with BLAST against viral databases. Purified B-cells from two 
      CLL patients, with and without stimulation by phorbol-esters, were sequenced 
      using Illumina technology to achieve depth of sequencing. Burrows-Wheeler Aligner 
      mapping and BLAST searches were used for the Illumina data. Pyrosequencing 
      resulted in about 400 000 reads per sample. No viral candidate could be found. 
      Illumina single-end sequencing for 115 cycles yielded an average of 26 +/- 2.5 
      million filtered reads per sample, of which 2.2 +/- 0.6 million remained unmapped 
      to human references. BLAST searches of these reads against viral and human 
      databases assigned nine reads to an Epstein-Barr virus origin, in one sample 
      following phorbol-ester stimulation. Other reads showing a putative viral origin 
      were dismissed after further analysis. Despite an in-depth analysis of the CLL 
      transcriptome reaching more than 100 million sequences, we have not found 
      evidence for a putative viral candidate in CLL.
CI  - (c) 2012 Blackwell Publishing Ltd.
FAU - Rego, Natalia
AU  - Rego N
AD  - Institut Pasteur de Montevideo, Montevideo, Uruguay.
FAU - Bianchi, Sergio
AU  - Bianchi S
FAU - Moreno, Pilar
AU  - Moreno P
FAU - Persson, Helena
AU  - Persson H
FAU - Kvist, Anders
AU  - Kvist A
FAU - Pena, Alvaro
AU  - Pena A
FAU - Oppezzo, Pablo
AU  - Oppezzo P
FAU - Naya, Hugo
AU  - Naya H
FAU - Rovira, Carlos
AU  - Rovira C
FAU - Dighiero, Guillermo
AU  - Dighiero G
FAU - Pritsch, Otto
AU  - Pritsch O
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120410
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
SB  - IM
MH  - Aged
MH  - B-Lymphocytes/metabolism/*virology
MH  - Female
MH  - Humans
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics/metabolism/*virology
MH  - Male
MH  - Middle Aged
MH  - *Transcriptome
PMC - PMC7161782
COIS- The authors reported no potential conflicts of interest.
EDAT- 2012/04/12 06:00
MHDA- 2012/07/24 06:00
PMCR- 2020/04/16
CRDT- 2012/04/12 06:00
PHST- 2011/12/22 00:00 [received]
PHST- 2012/03/06 00:00 [accepted]
PHST- 2012/04/12 06:00 [entrez]
PHST- 2012/04/12 06:00 [pubmed]
PHST- 2012/07/24 06:00 [medline]
PHST- 2020/04/16 00:00 [pmc-release]
AID - BJH9116 [pii]
AID - 10.1111/j.1365-2141.2012.09116.x [doi]
PST - ppublish
SO  - Br J Haematol. 2012 Jun;157(6):709-17. doi: 10.1111/j.1365-2141.2012.09116.x. 
      Epub 2012 Apr 10.