PMID- 22489689
OWN - NLM
STAT- MEDLINE
DCOM- 20120807
LR  - 20220331
IS  - 0028-2685 (Print)
IS  - 0028-2685 (Linking)
VI  - 59
IP  - 4
DP  - 2012
TI  - Fluorescence in situ hybridization assay detects upper urinary tract transitional 
      cell carcinoma in patients with asymptomatic hematuria and negative urine 
      cytology.
PG  - 355-60
LID - 10.4149/neo_2012_046 [doi]
AB  - We evaluated the performance of a multiprobe FISH (fluorescence in situ 
      hybridization) assay for noninvasive detection of upper urinary tract 
      transitional cell carcinoma (UUT-TCC) in patients with asymptomatic hematuria and 
      negative urine cytology. Voided urine samples from 285 patients with asymptomatic 
      hematuria and negative urine cytology were prospectively analyzed by FISH 
      technique. FISH assays were performed to detect chromosomal changes frequently 
      associated with TCC, including aneuploidy of chromosomes 3, 7 and 17, and loss of 
      the 9p21 locus. Eleven (3.9%) had a positive FISH result. Of the 11 patients, 
      nine (81.8%) were found to have a TCC of the upper urinary tract, while no 
      patients with negative FISH findings were found to have UUT-TCC. In this selected 
      cohort, the sensitivity and specificity of FISH for the detection of UUT-TCC was 
      100% and 99.3%, respectively. Our preliminary data suggest that the clinical 
      utility of FISH assay of chromosomes 3, 7, 9, and 17 as a noninvasive ancillary 
      test for the diagnosis of UUT-TCC in a selected patient population with 
      asymptomatic hematuria and negative urine cytology and by significant high 
      sensitivity and specificity may be a reliable diagnostic approach for early 
      detection of UUT-TCC patients. Further larger prospective and multicenter trials 
      are needed to confirm our results.
FAU - Huang, W T
AU  - Huang WT
AD  - Department of Urology, The Third Affiliated Hospital, Sun Yat-sen University, 
      Guangzhou, 510630, China.
FAU - Li, L Y
AU  - Li LY
FAU - Pang, J
AU  - Pang J
FAU - Ruan, X X
AU  - Ruan XX
FAU - Sun, Q P
AU  - Sun QP
FAU - Yang, W J
AU  - Yang WJ
FAU - Gao, X
AU  - Gao X
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Slovakia
TA  - Neoplasma
JT  - Neoplasma
JID - 0377266
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Transitional Cell/*diagnosis/genetics/urine
MH  - *Cytodiagnosis
MH  - Female
MH  - Hematuria/*genetics/urine
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Prognosis
MH  - Prospective Studies
MH  - Urinary Bladder Neoplasms/*diagnosis/genetics/urine
MH  - Urine/*cytology
EDAT- 2012/04/12 06:00
MHDA- 2012/08/08 06:00
CRDT- 2012/04/12 06:00
PHST- 2012/04/12 06:00 [entrez]
PHST- 2012/04/12 06:00 [pubmed]
PHST- 2012/08/08 06:00 [medline]
AID - 10.4149/neo_2012_046 [doi]
PST - ppublish
SO  - Neoplasma. 2012;59(4):355-60. doi: 10.4149/neo_2012_046.