PMID- 22489946
OWN - NLM
STAT- MEDLINE
DCOM- 20120814
LR  - 20181201
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Linking)
VI  - 79
IP  - 5
DP  - 2012 May
TI  - Assessment of fidelity and utility of the whole-genome amplification for the 
      clinical tests offered in a histocompatibility and immunogenetics laboratory.
PG  - 372-9
LID - 10.1111/j.1399-0039.2012.01857.x [doi]
AB  - Increasing emphasis on the use of molecular tests in a histocompatibility and 
      immunogenetics laboratory (HIL) poses a potential problem of lack of sufficient 
      DNA to perform multiple genetic analyses. In this study, we report the 
      feasibility, fidelity and utility of multiple displacement amplification (MDA) 
      method to perform whole-genome amplification (WGA) to generate DNA specimens that 
      can be analyzed by multiple molecular techniques and can be used for different 
      clinical tests offered by an HIL. The MDA-generated DNA when compared with the 
      native DNA showed 100% congruency in genotyping of 37 genes/loci using multiple 
      downstream molecular techniques: sequence-based typing and sequence-specific 
      primer-based typing for 5 human leukocyte antigen (HLA) class I and II genes 
      (HLA-A, B, C, DRB1 and DQB1), luminex-based sequence-specific oligonucleotide 
      (SSO) genotyping for a panel of 16 killer immunoglobulin-like receptor (KIR) 
      genes and automated fragment size analysis for a panel of 15 short tandem repeat 
      (STR) loci and amelogenin gene. For post-allogeneic hematopoietic cell 
      transplantation (HCT) chimerism analysis, MDA-generated DNA appeared useful for 
      enriching pre-transplant DNA but not for enriching post-transplant chimeric DNA. 
      Overall, our results show that MDA-based WGA could generate DNA of high yield and 
      fidelity that can be used for various clinical tests and research purposes.
CI  - (c) 2012 John Wiley & Sons A/S.
FAU - Khan, F
AU  - Khan F
AD  - Tissue Typing Laboratory, Calgary Laboratory Services, Calgary, Alberta, Canada. 
      fkhan@ucalgary.ca
FAU - Liacini, A
AU  - Liacini A
FAU - Arora, E
AU  - Arora E
FAU - Wang, S
AU  - Wang S
FAU - Assad, M
AU  - Assad M
FAU - Doulla, J
AU  - Doulla J
FAU - Faridi, R M
AU  - Faridi RM
FAU - Berka, N
AU  - Berka N
LA  - eng
PT  - Journal Article
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (Amelogenin)
RN  - 0 (DNA Primers)
RN  - 0 (HLA Antigens)
RN  - 0 (Receptors, KIR)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Amelogenin/genetics/immunology
MH  - DNA/*analysis/genetics
MH  - DNA Fingerprinting/*methods
MH  - DNA Primers
MH  - Gene Expression Profiling
MH  - Genetic Loci
MH  - *Genome, Human
MH  - Genome-Wide Association Study
MH  - HLA Antigens/genetics/immunology
MH  - Hematopoietic Stem Cell Transplantation
MH  - Histocompatibility Testing
MH  - Humans
MH  - Nucleic Acid Amplification Techniques/*methods/statistics & numerical data
MH  - Receptors, KIR/genetics/immunology
MH  - Sequence Analysis, DNA
MH  - Tandem Repeat Sequences
MH  - Transplantation, Homologous
EDAT- 2012/04/12 06:00
MHDA- 2012/08/15 06:00
CRDT- 2012/04/12 06:00
PHST- 2012/04/12 06:00 [entrez]
PHST- 2012/04/12 06:00 [pubmed]
PHST- 2012/08/15 06:00 [medline]
AID - 10.1111/j.1399-0039.2012.01857.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2012 May;79(5):372-9. doi: 10.1111/j.1399-0039.2012.01857.x.