PMID- 22490401
OWN - NLM
STAT- MEDLINE
DCOM- 20120803
LR  - 20220321
IS  - 2542-5641 (Electronic)
IS  - 0366-6999 (Linking)
VI  - 125
IP  - 3
DP  - 2012 Feb
TI  - Correlation of epidermal growth factor receptor overexpression with increased 
      epidermal growth factor receptor gene copy number in esophageal squamous cell 
      carcinomas.
PG  - 450-4
AB  - BACKGROUND: Esophageal squamous cell carcinoma (ESCC) is one of the most frequent 
      malignancies in China and epidermal growth factor receptor (EGFR) is widely 
      distributed in human epithelial cell membrane. The aim of this study was to 
      investigate the protein overexpression and gene copy number of EGFR in ESCC, and 
      help to identify patients who may benefit from EGFR targeted therapies. METHODS: 
      Immunohistochemistry (IHC) was performed to analyze the expression of EGFR in 105 
      cases of ESCC, 16 cases of squamous epithelial atypical hyperplasia, and 11 cases 
      of normal esophageal tissue. Fluorescence in situ hybridization (FISH) was 
      performed to analyze the gene copy number in 80 cases of ESCC, eight cases of 
      squamous epithelial atypical hyperplasia, and eight samples of normal esophageal 
      tissue. RESULTS: The IHC-positive rates of EGFR in 105 cases of ESCC, 16 cases of 
      squamous epithelial atypical hyperplasia, and 11 normal esophageal tissues were 
      97% (102/105), 44% (7/16), and 18% (2/11) respectively. The difference in the 
      expression of EGFR among different esophageal tissue groups had statistically 
      significance (P < 0.05). Among the 105 cases of ESCC, overexpression of EGFR was 
      found in 90 cases (86%), of which 55 cases scored 3+ for EGFR staining and 35 
      cases scored 2+. In ESCC, the expression of EGFR was significantly correlated 
      with depth of invasion and TNM stage (P < 0.05), but not with other parameters. 
      The FISH-positive rates of EGFR in 80 cases of ESCC, the eight cases of squamous 
      epithelial atypical hyperplasia, and eight samples of normal esophageal tissue 
      were 31.3% (25/80), 0 (0/8) and 0 (0/8) respectively. In ESCC, EGFR gene 
      amplification was found in 17 (21%) cases, high polysomy in 8 (10%) cases, disomy 
      in 34 cases, low trisomy in 17 cases, and high trisomy in four cases. EGFR 
      FISH-positive was significantly correlated with depth of invasion and lymph node 
      metastasis (P < 0.05). EGFR FISH-positive was significantly associated with 
      overexpression of EGFR. CONCLUSION: Protein overexpression and/or increased gene 
      copy number of EGFR is common in ESCC, and EGFR targeted therapy may be 
      appropriate for ESCC patients.
FAU - Yang, Yan-Li
AU  - Yang YL
AD  - Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China.
FAU - Xu, Kan-Lun
AU  - Xu KL
FAU - Zhou, Yan
AU  - Zhou Y
FAU - Gao, Xin
AU  - Gao X
FAU - Chen, Li-Rong
AU  - Chen LR
LA  - eng
PT  - Journal Article
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Squamous Cell
MH  - ErbB Receptors/genetics/*metabolism
MH  - Esophageal Neoplasms/genetics/*metabolism
MH  - Female
MH  - Gene Dosage/genetics
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
EDAT- 2012/04/12 06:00
MHDA- 2012/08/04 06:00
CRDT- 2012/04/12 06:00
PHST- 2012/04/12 06:00 [entrez]
PHST- 2012/04/12 06:00 [pubmed]
PHST- 2012/08/04 06:00 [medline]
PST - ppublish
SO  - Chin Med J (Engl). 2012 Feb;125(3):450-4.