PMID- 22490469 OWN - NLM STAT- MEDLINE DCOM- 20121001 LR - 20120612 IS - 1096-0309 (Electronic) IS - 0003-2697 (Linking) VI - 426 IP - 2 DP - 2012 Jul 15 TI - Expression of functional full-length hSRC-1 in eukaryotic cells using modified vaccinia virus Ankara and baculovirus. PG - 106-8 LID - 10.1016/j.ab.2012.04.006 [doi] AB - Purified protein expression level and quality are contingent upon specific host expression systems. This differential production is particularly observed for proteins of high molecular weight, hampering further structural studies. We developed an expression method aimed at producing proteins in Escherichia coli, insect, and mammalian systems. Our novel protocol was used to produce in large scale the full-length 160-kDa steroid receptor coactivator 1 (SRC-1), a coregulator of nuclear receptors. The results indicate that we can produce biologically active human SRC-1 in mammalian and insect cells in large scale. CI - Copyright (c) 2012 Elsevier Inc. All rights reserved. FAU - Osz, Judit AU - Osz J AD - Institut de Genetique et de Biologie Moleculaire et Cellulaire, Institut National de Sante et de Recherche Medicale U964/Centre National de Recherche Scientifique UMR 7104/Universite de Strasbourg, 67404 Illkirch, France. FAU - Pradeau-Aubreton, Karine AU - Pradeau-Aubreton K FAU - Drillien, Robert AU - Drillien R FAU - Troffer-Charlier, Nathalie AU - Troffer-Charlier N FAU - Kolb-Cheynel, Isabelle AU - Kolb-Cheynel I FAU - Poterszman, Arnaud AU - Poterszman A FAU - Ruff, Marc AU - Ruff M FAU - Moras, Dino AU - Moras D FAU - Rochel, Natacha AU - Rochel N LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20120407 PL - United States TA - Anal Biochem JT - Analytical biochemistry JID - 0370535 RN - 0 (Recombinant Proteins) RN - EC 2.3.1.48 (Nuclear Receptor Coactivator 1) SB - IM MH - Animals MH - Baculoviridae/*genetics MH - Cell Line MH - Cricetinae MH - Genetic Vectors/*metabolism MH - Humans MH - Nuclear Receptor Coactivator 1/*biosynthesis/genetics MH - Recombinant Proteins/biosynthesis/genetics MH - Transfection MH - Vaccinia virus/*genetics EDAT- 2012/04/12 06:00 MHDA- 2012/10/02 06:00 CRDT- 2012/04/12 06:00 PHST- 2012/01/27 00:00 [received] PHST- 2012/03/09 00:00 [revised] PHST- 2012/04/02 00:00 [accepted] PHST- 2012/04/12 06:00 [entrez] PHST- 2012/04/12 06:00 [pubmed] PHST- 2012/10/02 06:00 [medline] AID - S0003-2697(12)00210-2 [pii] AID - 10.1016/j.ab.2012.04.006 [doi] PST - ppublish SO - Anal Biochem. 2012 Jul 15;426(2):106-8. doi: 10.1016/j.ab.2012.04.006. Epub 2012 Apr 7.