PMID- 22493336
OWN - NLM
STAT- MEDLINE
DCOM- 20120724
LR  - 20211021
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Print)
IS  - 0095-1137 (Linking)
VI  - 50
IP  - 6
DP  - 2012 Jun
TI  - Multicenter evaluation of the Staphylococcus QuickFISH method for simultaneous 
      identification of Staphylococcus aureus and coagulase-negative staphylococci 
      directly from blood culture bottles in less than 30 minutes.
PG  - 1994-8
LID - 10.1128/JCM.00225-12 [doi]
AB  - A novel rapid peptide nucleic acid fluorescence in situ hybridization (FISH) 
      method, Staphylococcus QuickFISH, for the direct detection of Staphylococcus 
      species from positive blood culture bottles was evaluated in a multicenter 
      clinical study. The method utilizes a microscope slide with predeposited 
      positive- and negative-control organisms and a self-reporting 15-min 
      hybridization step, which eliminates the need for a wash step. Five clinical 
      laboratories tested 722 positive blood culture bottles containing gram-positive 
      cocci in clusters. The sensitivities for detection of Staphylococcus aureus and 
      coagulase-negative staphylococci (CoNS) were 99.5% (217/218) and 98.8% (487/493), 
      respectively, and the combined specificity of the assay was 89.5% (17/19). The 
      combined positive and negative predictive values of the assay were 99.7% 
      (696/698) and 70.8% (17/24), respectively. Studies were also performed on spiked 
      cultures to establish the specificity and performance sensitivity of the method. 
      Staphylococcus QuickFISH has a turnaround time (TAT) of <30 min and a hands-on 
      time (HOT) of <5 min. The ease and speed of the method have the potential to 
      improve the accuracy of therapeutic intervention by providing S. aureus/CoNS 
      identification simultaneously with Gram stain results.
FAU - Deck, Melissa K
AU  - Deck MK
AD  - AdvanDx Inc., Woburn, Massachusetts, USA.
FAU - Anderson, Erica S
AU  - Anderson ES
FAU - Buckner, Rebecca J
AU  - Buckner RJ
FAU - Colasante, Georgia
AU  - Colasante G
FAU - Coull, James M
AU  - Coull JM
FAU - Crystal, Benjamin
AU  - Crystal B
FAU - Della Latta, Phyllis
AU  - Della Latta P
FAU - Fuchs, Martin
AU  - Fuchs M
FAU - Fuller, Deanna
AU  - Fuller D
FAU - Harris, Will
AU  - Harris W
FAU - Hazen, Kevin
AU  - Hazen K
FAU - Klimas, Lisa L
AU  - Klimas LL
FAU - Lindao, Daniel
AU  - Lindao D
FAU - Meltzer, Michelle C
AU  - Meltzer MC
FAU - Morgan, Margie
AU  - Morgan M
FAU - Shepard, Janeen
AU  - Shepard J
FAU - Stevens, Sharon
AU  - Stevens S
FAU - Wu, Fann
AU  - Wu F
FAU - Fiandaca, Mark J
AU  - Fiandaca MJ
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Multicenter Study
DEP - 20120404
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
SB  - IM
MH  - Bacteremia/*diagnosis/microbiology
MH  - Bacteriological Techniques/*methods
MH  - Blood/*microbiology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Molecular Diagnostic Techniques/*methods
MH  - Predictive Value of Tests
MH  - Sensitivity and Specificity
MH  - Staphylococcal Infections/*diagnosis/microbiology
MH  - Staphylococcus/genetics/*isolation & purification
PMC - PMC3372102
EDAT- 2012/04/12 06:00
MHDA- 2012/07/25 06:00
PMCR- 2012/12/01
CRDT- 2012/04/12 06:00
PHST- 2012/04/12 06:00 [entrez]
PHST- 2012/04/12 06:00 [pubmed]
PHST- 2012/07/25 06:00 [medline]
PHST- 2012/12/01 00:00 [pmc-release]
AID - JCM.00225-12 [pii]
AID - 00225-12 [pii]
AID - 10.1128/JCM.00225-12 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2012 Jun;50(6):1994-8. doi: 10.1128/JCM.00225-12. Epub 2012 Apr 
      4.