PMID- 22494516
OWN - NLM
STAT- MEDLINE
DCOM- 20121009
LR  - 20191210
IS  - 1873-264X (Electronic)
IS  - 0731-7085 (Linking)
VI  - 66
DP  - 2012 Jul
TI  - Simultaneous quantification of coumarins, flavonoids and limonoids in Fructus 
      Citri Sarcodactylis by high performance liquid chromatography coupled with diode 
      array detector.
PG  - 170-5
LID - 10.1016/j.jpba.2012.03.041 [doi]
AB  - A high performance liquid chromatography coupled with diode array detector 
      (HPLC-DAD) method was developed for simultaneous quantification of eleven major 
      bioactive components including six coumarins, three flavonoids and two limonoids 
      in Fructus Citri Sarcodactylis. The analysis was performed on a Cosmosil 5 
      C(18)-MS-II column (4.6 mm x 250 mm, 5 mum) with water-acetonitrile gradient 
      elution. The method was validated in terms of linearity, sensitivity, precision, 
      stability and accuracy. It was found that the calibration curves for all analytes 
      showed good linearity (R(2)>0.9993) within the test ranges. The overall limit of 
      detection (LOD) and limit of quantification (LOQ) were less than 3.0 and 10.2 ng. 
      The relative standard deviations (RSDs) for intra- and inter-day repeatability 
      were not more than 4.99% and 4.92%, respectively. The sample was stable for at 
      least 48 h. The spike recoveries of eleven components were 95.1-104.9%. The 
      established method was successfully applied to determine eleven components in 
      three samples from different locations. The results showed that the newly 
      developed HPLC-DAD method was linear, sensitive, precise and accurate, and could 
      be used for quality control of Fructus Citri Sarcodactylis.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Chu, Jun
AU  - Chu J
AD  - State Key Laboratory of Quality Research in Chinese Medicine (University of 
      Macau), Macao.
FAU - Li, Song-Lin
AU  - Li SL
FAU - Yin, Zhi-Qi
AU  - Yin ZQ
FAU - Ye, Wen-Cai
AU  - Ye WC
FAU - Zhang, Qing-Wen
AU  - Zhang QW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20120328
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Coumarins)
RN  - 0 (Flavonoids)
RN  - 0 (Limonins)
SB  - IM
MH  - Calibration
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Citrus/*chemistry
MH  - Coumarins/*analysis/chemistry/isolation & purification
MH  - Flavonoids/*analysis/chemistry/isolation & purification
MH  - Limit of Detection
MH  - Limonins/*analysis/chemistry/isolation & purification
MH  - Quality Control
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Time Factors
EDAT- 2012/04/13 06:00
MHDA- 2012/10/10 06:00
CRDT- 2012/04/13 06:00
PHST- 2012/01/06 00:00 [received]
PHST- 2012/03/19 00:00 [revised]
PHST- 2012/03/19 00:00 [accepted]
PHST- 2012/04/13 06:00 [entrez]
PHST- 2012/04/13 06:00 [pubmed]
PHST- 2012/10/10 06:00 [medline]
AID - S0731-7085(12)00164-1 [pii]
AID - 10.1016/j.jpba.2012.03.041 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2012 Jul;66:170-5. doi: 10.1016/j.jpba.2012.03.041. Epub 
      2012 Mar 28.