PMID- 22496599
OWN - NLM
STAT- MEDLINE
DCOM- 20120702
LR  - 20211021
IS  - 1466-1861 (Electronic)
IS  - 0962-9351 (Print)
IS  - 0962-9351 (Linking)
VI  - 2012
DP  - 2012
TI  - Activation of peroxisome proliferator-activated receptor-gamma by glitazones 
      reduces the expression and release of monocyte chemoattractant protein-1 in human 
      mesothelial cells.
PG  - 217696
LID - 10.1155/2012/217696 [doi]
LID - 217696
AB  - Human peritoneal mesothelial cells (MC) play an important role in inflammatory 
      processes of the peritoneal cavity by producing various cytokines and chemokines, 
      such as monocyte chemoattractant protein-1 (MCP-1). The present study was 
      designed to assess the effect of the peroxisome proliferator-activated 
      receptor-gamma- (PPARgamma-) activator rosiglitazone on the mesothelial MCP-1 
      expression and release. Primary cultures of MC were obtained from omental tissue. 
      MCP-1 antigen concentrations were measured in the cell supernatant by ELISA and 
      MCP-1 mRNA levels by real-time polymerase chain reaction. The presence of PPARgamma 
      on MC was assayed in a Western Blot analysis. MC constitutively express PPARgamma. 
      Activation of this receptor via rosiglitazone (0,1-10 mumol/L) resulted in 
      significantly reduced amounts of mesothelial MCP-1 release as well as MCP-1 mRNA. 
      The use of the PPARgamma inhibitor GW-9662 could completely prevent the rosiglitazone 
      effects. Rosiglitazone was also effective in reducing TNFalpha-induced enhanced 
      secretion of MCP-1. Our findings indicate that glitazones are effective in 
      reducing constitutive and TNFalpha-stimulated mesothelial MCP-1 mRNA expression and 
      release.
FAU - Sauter, Matthias
AU  - Sauter M
AD  - Medizinische Poliklinik-Innenstadt, Klinikum der Universitaet Muenchen, Muenchen, 
      Germany. matthias.sauter@med.uni-muenchen.de
FAU - Kastenmuller, Kathrin
AU  - Kastenmuller K
FAU - Belling, Franziska
AU  - Belling F
FAU - Wornle, Markus
AU  - Wornle M
FAU - Ladurner, Roland
AU  - Ladurner R
FAU - Mussack, Thomas
AU  - Mussack T
FAU - Sitter, Thomas
AU  - Sitter T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120207
PL  - United States
TA  - Mediators Inflamm
JT  - Mediators of inflammation
JID - 9209001
RN  - 0 (Chemokine CCL2)
RN  - 0 (PPAR gamma)
RN  - 0 (Thiazolidinediones)
RN  - 05V02F2KDG (Rosiglitazone)
SB  - IM
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Epithelial Cells/*drug effects/*metabolism
MH  - Epithelium/*drug effects/*metabolism
MH  - Humans
MH  - PPAR gamma/genetics/*metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Rosiglitazone
MH  - Thiazolidinediones/*pharmacology
PMC - PMC3306974
EDAT- 2012/04/13 06:00
MHDA- 2012/07/03 06:00
PMCR- 2012/02/07
CRDT- 2012/04/13 06:00
PHST- 2011/08/09 00:00 [received]
PHST- 2011/10/30 00:00 [accepted]
PHST- 2012/04/13 06:00 [entrez]
PHST- 2012/04/13 06:00 [pubmed]
PHST- 2012/07/03 06:00 [medline]
PHST- 2012/02/07 00:00 [pmc-release]
AID - 10.1155/2012/217696 [doi]
PST - ppublish
SO  - Mediators Inflamm. 2012;2012:217696. doi: 10.1155/2012/217696. Epub 2012 Feb 7.