PMID- 22498881
OWN - NLM
STAT- MEDLINE
DCOM- 20130110
LR  - 20211021
IS  - 1876-7737 (Electronic)
IS  - 1874-3919 (Print)
IS  - 1874-3919 (Linking)
VI  - 75
IP  - 16
DP  - 2012 Aug 30
TI  - Mass spectrometry imaging and profiling of single cells.
PG  - 5036-5051
LID - S1874-3919(12)00150-9 [pii]
LID - 10.1016/j.jprot.2012.03.017 [doi]
AB  - Mass spectrometry imaging and profiling of individual cells and subcellular 
      structures provide unique analytical capabilities for biological and biomedical 
      research, including determination of the biochemical heterogeneity of cellular 
      populations and intracellular localization of pharmaceuticals. Two mass 
      spectrometry technologies-secondary ion mass spectrometry (SIMS) and matrix 
      assisted laser desorption/ionization mass spectrometry (MALDI MS)-are most often 
      used in micro-bioanalytical investigations. Recent advances in ion probe 
      technologies have increased the dynamic range and sensitivity of analyte 
      detection by SIMS, allowing two- and three-dimensional localization of analytes 
      in a variety of cells. SIMS operating in the mass spectrometry imaging (MSI) mode 
      can routinely reach spatial resolutions at the submicron level; therefore, it is 
      frequently used in studies of the chemical composition of subcellular structures. 
      MALDI MS offers a large mass range and high sensitivity of analyte detection. It 
      has been successfully applied in a variety of single-cell and organelle profiling 
      studies. Innovative instrumentation such as scanning microprobe MALDI and mass 
      microscope spectrometers enables new subcellular MSI measurements. Other 
      approaches for MS-based chemical imaging and profiling include those based on 
      near-field laser ablation and inductively-coupled plasma MS analysis, which offer 
      complementary capabilities for subcellular chemical imaging and profiling.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Lanni, Eric J
AU  - Lanni EJ
AD  - Department of Chemistry and the Beckman Institute of Science and Technology, 
      University of Illinois, Urbana IL 61801, USA.
FAU - Rubakhin, Stanislav S
AU  - Rubakhin SS
AD  - Department of Chemistry and the Beckman Institute of Science and Technology, 
      University of Illinois, Urbana IL 61801, USA.
FAU - Sweedler, Jonathan V
AU  - Sweedler JV
AD  - Department of Chemistry and the Beckman Institute of Science and Technology, 
      University of Illinois, Urbana IL 61801, USA. Electronic address: 
      jsweedle@illinois.edu.
LA  - eng
GR  - P30 DA018310/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20120329
PL  - Netherlands
TA  - J Proteomics
JT  - Journal of proteomics
JID - 101475056
SB  - IM
MH  - Animals
MH  - Diagnostic Imaging/methods
MH  - Humans
MH  - Mass Spectrometry/*methods
MH  - *Metabolome
MH  - Proteomics/methods
MH  - Single-Cell Analysis/*methods
MH  - Spectrometry, Mass, Secondary Ion/methods
PMC - PMC3419297
MID - NIHMS372536
COIS- The authors declare they have no conflicts of interest.
EDAT- 2012/04/14 06:00
MHDA- 2013/01/11 06:00
PMCR- 2013/08/30
CRDT- 2012/04/14 06:00
PHST- 2011/12/21 00:00 [received]
PHST- 2012/03/08 00:00 [revised]
PHST- 2012/03/13 00:00 [accepted]
PHST- 2012/04/14 06:00 [entrez]
PHST- 2012/04/14 06:00 [pubmed]
PHST- 2013/01/11 06:00 [medline]
PHST- 2013/08/30 00:00 [pmc-release]
AID - S1874-3919(12)00150-9 [pii]
AID - 10.1016/j.jprot.2012.03.017 [doi]
PST - ppublish
SO  - J Proteomics. 2012 Aug 30;75(16):5036-5051. doi: 10.1016/j.jprot.2012.03.017. 
      Epub 2012 Mar 29.