PMID- 22499392 OWN - NLM STAT- MEDLINE DCOM- 20121127 LR - 20151119 IS - 1552-4965 (Electronic) IS - 1549-3296 (Linking) VI - 100 IP - 9 DP - 2012 Sep TI - The in vivo osteogenesis of Mg or Zr-modified silicate-based bioceramic spheres. PG - 2269-77 LID - 10.1002/jbm.a.34161 [doi] AB - The use of bioactive microspheres as bone filling materials has received much attention due to their ability to fill the bone defects with irregular and complex shapes and sizes. Divalent Mg(2+) modified silicate-based diopside (DIOP: CaMgSi(2)O(6)) and tetravalent Zr(4+) modified silicate-based baghdadite (BAGD: Ca(3)ZrSi(2)O(9)) ceramics have shown excellent in vitro bioactivity for potential bone repair application. However, their in vivo osteogenesis has not been systematically investigated. The aim of this study is to prepare DIOP and BAGD ceramic microspheres and investigate their in vivo osteogenesis. DIOP and BAGD ceramic spheres with loose microstructure were successfully prepared. The dissolution ability of two silicate-based bioceramics was investigated by testing the release of SiO 44- ions after soaking them in phosphate buffered saline. The ceramic spheres were implanted into supracondylar site of the femur defects in Wistar rats and the degree of in vivo osteogenesis was evaluated by hematoxylin and eosin (H and E), Safranin O staining, tartrate-resistant acid phosphatase (TRAP) staining, and immunohistochemistry (type I collagen: Col I, osteopontin: OPN) analyses. The results have shown that BAGD spheres induced a higher rate of new bone formation in the defects than did DIOP and beta-tricalcium phosphate (beta-TCP) spheres. Immunohistochemical analysis showed greater expression of Col I and OPN in BAGD group compared to DIOP and beta-TCP groups. The study indicates different ion modification playing an important role to regulate the in vivo osteogenesis of silicate-based bioceramics. BAGD spheres are a promising bone filler material due to their significantly enhanced osteogenesis, compared to beta-TCP spheres. CI - Copyright (c) 2012 Wiley Periodicals, Inc. FAU - Luo, Tao AU - Luo T AD - The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, People's Republic of China. FAU - Wu, Chengtie AU - Wu C FAU - Zhang, Yufeng AU - Zhang Y LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20120412 PL - United States TA - J Biomed Mater Res A JT - Journal of biomedical materials research. Part A JID - 101234237 RN - 0 (Bone Substitutes) RN - 0 (Silicates) RN - 1343-98-2 (Silicic Acid) RN - 14483-19-3 (diopside) RN - C6V6S92N3C (Zirconium) RN - I38ZP9992A (Magnesium) SB - IM MH - Animals MH - *Bone Regeneration MH - Bone Substitutes/*chemistry/metabolism MH - Ceramics/*chemistry/metabolism MH - Female MH - Femur/injuries/*physiology/ultrastructure MH - Magnesium/*chemistry/metabolism MH - Rats MH - Rats, Wistar MH - Silicates/*chemistry/metabolism MH - Silicic Acid/chemistry/metabolism MH - Zirconium/*chemistry/metabolism EDAT- 2012/04/14 06:00 MHDA- 2012/12/10 06:00 CRDT- 2012/04/14 06:00 PHST- 2011/10/11 00:00 [received] PHST- 2012/01/30 00:00 [revised] PHST- 2012/03/02 00:00 [accepted] PHST- 2012/04/14 06:00 [entrez] PHST- 2012/04/14 06:00 [pubmed] PHST- 2012/12/10 06:00 [medline] AID - 10.1002/jbm.a.34161 [doi] PST - ppublish SO - J Biomed Mater Res A. 2012 Sep;100(9):2269-77. doi: 10.1002/jbm.a.34161. Epub 2012 Apr 12.