PMID- 22499464
OWN - NLM
STAT- MEDLINE
DCOM- 20120906
LR  - 20240317
IS  - 1760-4788 (Electronic)
IS  - 1279-7707 (Linking)
VI  - 16
IP  - 4
DP  - 2012 Apr
TI  - Effects of genistein and daidzein on hippocampus neuronal cell proliferation and 
      BDNF expression in H19-7 neural cell line.
PG  - 389-94
AB  - OBJECTIVES: Estrogen replacement therapy (ERT) reduces the risk of Alzheimer's 
      disease and symptoms in postmenopausal and elderly women. However, ERT is 
      associated with increased risk of uterine and breast cancer. Dietary 
      phytoestrogens have been suggested as a potential alternative to ERT, while 
      little information is available regarding the effects and the underlying 
      mechanisms of such treatment on central neuron function. The present study aimed 
      to determine the effects of phytoestrogens including genistein and daidzein on 
      the proliferation and survival of the hippocampus neural cells, which are of 
      importance in learning and memory function. MEASUREMENTS: H19-7/IGF-IR neural 
      cell line was cultured in DMEM absented of serum for 72 h, and treated with 
      various concentrations of genistein, daidzein or 17beta-estradiol. Neuronal cell 
      viability and proliferation were determined by MTT and BrdU assay, respectively 
      Cell cycle analysis was performed using flow cytometry. The effects of genistein 
      and daidzein on brain-derived neurotrophic factor (BDNF) mRNA and protein 
      expression were determined by RT-PCR and ELISA, respectively. The effect of Trk 
      receptors inhibitor on genistein and daidzein - induced hippocampus neuronal cell 
      proliferation was also examined. RESULTS: 17beta-estradiol, genistein and daidzein 
      ranged from 20 nM to 2000 nM significantly promoted hippocampus neuronal cell 
      viability and proliferation. Similar to the effect of 17beta-estradiol, genistein 
      and daidzein induced an increase in the percentage of cells in S phase. Genistein 
      and daidzein significantly increased the expression of BDNF mRNA and protein 
      levels. The effect of genistien and daidzein on hippocampus neuronal 
      proliferation was blocked by K252a, a selective Trk receptors inhibitor. 
      CONCLUSION: This study concluded that genistein and daidzein improved hippocampus 
      neuronal cell viability and proliferation in vitro. These neuroprotective effects 
      might be mediated by BDNF-Trk pathway.
FAU - Pan, M
AU  - Pan M
AD  - Department of Nutrition, Guangdong Academy of Medical Sciences, Guangdong General 
      Hospital, Guangzhou, P R China. meixpan@yahoo.com.cn
FAU - Han, H
AU  - Han H
FAU - Zhong, C
AU  - Zhong C
FAU - Geng, Q
AU  - Geng Q
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - J Nutr Health Aging
JT  - The journal of nutrition, health & aging
JID - 100893366
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Isoflavones)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Phytoestrogens)
RN  - 4TI98Z838E (Estradiol)
RN  - 6287WC5J2L (daidzein)
RN  - DH2M523P0H (Genistein)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics/metabolism
MH  - Cell Cycle/drug effects
MH  - Cell Line
MH  - Cell Proliferation/*drug effects
MH  - Cell Survival/drug effects
MH  - Estradiol/pharmacology
MH  - Estrogen Replacement Therapy/adverse effects/methods
MH  - Gene Expression Regulation
MH  - Genistein/*pharmacology
MH  - Hippocampus/*cytology/drug effects
MH  - Isoflavones/*pharmacology
MH  - Neurons/cytology/*drug effects
MH  - Neuroprotective Agents/pharmacology
MH  - Phytoestrogens/*pharmacology
MH  - Rats
EDAT- 2012/04/14 06:00
MHDA- 2012/09/07 06:00
CRDT- 2012/04/14 06:00
PHST- 2012/04/14 06:00 [entrez]
PHST- 2012/04/14 06:00 [pubmed]
PHST- 2012/09/07 06:00 [medline]
AID - S1279-7707(23)01564-6 [pii]
AID - 10.1007/s12603-011-0140-3 [doi]
PST - ppublish
SO  - J Nutr Health Aging. 2012 Apr;16(4):389-94. doi: 10.1007/s12603-011-0140-3.