PMID- 22499984
OWN - NLM
STAT- MEDLINE
DCOM- 20120816
LR  - 20220321
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 53
IP  - 6
DP  - 2012 May 31
TI  - Spectral-domain optical coherence tomography as a noninvasive method to assess 
      damaged and regenerating adult zebrafish retinas.
PG  - 3126-38
LID - 10.1167/iovs.11-8895 [doi]
AB  - PURPOSE: These experiments assessed the ability of spectral-domain optical 
      coherence tomography (SD-OCT) to accurately represent the structural organization 
      of the adult zebrafish retina and reveal the dynamic morphologic changes during 
      either light-induced damage and regeneration of photoreceptors or ouabain-induced 
      inner retinal damage. METHODS: Retinas of control dark-adapted adult albino 
      zebrafish were compared with retinas subjected to 24 hours of constant intense 
      light and recovered for up to 8 weeks or ouabain-damaged retinas that recovered 
      for up to 3 weeks. Images were captured and the measurements of retinal 
      morphology were made by SD-OCT, and then compared with those obtained by 
      histology of the same eyes. RESULTS: Measurements between SD-OCT and histology 
      were very similar for the undamaged, damaged, and regenerating retinas. Axial 
      measurements of SD-OCT also revealed vitreal morphology that was not readily 
      visualized by histology. CONCLUSIONS: SD-OCT accurately represented retinal 
      lamination and photoreceptor loss and recovery during light-induced damage and 
      subsequent regeneration. SD-OCT was less accurate at detecting the inner nuclear 
      layer in ouabain-damaged retinas, but accurately detected the undamaged outer 
      nuclear layer. Thus, SD-OCT provides a noninvasive and quantitative method to 
      assess the morphology and the extent of damage and repair in the zebrafish 
      retina.
FAU - Bailey, Travis J
AU  - Bailey TJ
AD  - Department of Biological Sciences and the Center for Zebrafish Research, 
      University of Notre Dame, Notre Dame, IN 46556, USA.
FAU - Davis, Darin H
AU  - Davis DH
FAU - Vance, Joseph E
AU  - Vance JE
FAU - Hyde, David R
AU  - Hyde DR
LA  - eng
GR  - R01 EY018417/EY/NEI NIH HHS/United States
GR  - R01-EY018417/EY/NEI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20120531
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
SB  - IM
CIN - Invest Ophthalmol Vis Sci. 2012;53(11):7315. PMID: 23093132
MH  - Animals
MH  - Dark Adaptation
MH  - Disease Models, Animal
MH  - Photoreceptor Cells, Vertebrate/physiology
MH  - *Recovery of Function
MH  - *Regeneration
MH  - Retina/*physiology
MH  - Retinal Diseases/*pathology/physiopathology
MH  - Tomography, Optical Coherence/*methods
MH  - Zebrafish
PMC - PMC3383185
COIS- Disclosure: T.J. Bailey, None; D.H. Davis, Bioptigen Inc. (F, E); J.E. Vance, 
      Bioptigen Inc. (F, E); D.R. Hyde, None
EDAT- 2012/04/14 06:00
MHDA- 2012/08/17 06:00
PMCR- 2012/11/01
CRDT- 2012/04/14 06:00
PHST- 2012/04/14 06:00 [entrez]
PHST- 2012/04/14 06:00 [pubmed]
PHST- 2012/08/17 06:00 [medline]
PHST- 2012/11/01 00:00 [pmc-release]
AID - iovs.11-8895 [pii]
AID - 10.1167/iovs.11-8895 [doi]
PST - epublish
SO  - Invest Ophthalmol Vis Sci. 2012 May 31;53(6):3126-38. doi: 10.1167/iovs.11-8895.