PMID- 22499987
OWN - NLM
STAT- MEDLINE
DCOM- 20121024
LR  - 20240323
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 53
IP  - 9
DP  - 2012 Aug 7
TI  - Morphometric analysis of aqueous humor outflow structures with spectral-domain 
      optical coherence tomography.
PG  - 5198-207
LID - 10.1167/iovs.11-9229 [doi]
AB  - PURPOSE: To describe morphometric details of the human aqueous humor (AH) outflow 
      microvasculature visualized with 360-degree virtual castings during active AH 
      outflow in cadaver eyes and to compare these structures with corrosion casting 
      studies. METHODS: The conventional AH outflow pathways of donor eyes (n = 7) and 
      eyes in vivo (n = 3) were imaged with spectral-domain optical coherence 
      tomography (SD-OCT) and wide-bandwidth superluminescent diode array during active 
      AH outflow. Digital image contrast was adjusted to isolate AH microvasculature, 
      and images were viewed in a 3D viewer. Additional eyes (n = 3) were perfused with 
      mock AH containing fluorescent tracer microspheres to compare microvasculature 
      patterns. RESULTS: Observations revealed components of the conventional outflow 
      pathway from Schlemm's canal (SC) to the superficial intrascleral venous plexus 
      (ISVP). The superficial ISVP in both our study and corrosion casts were composed 
      of interconnected venules (10-50 mum) forming a hexagonal meshwork. Larger radial 
      arcades (50-100 mum) drained the region nearest SC and converged with larger 
      tortuous vessels (>100 mum). A 360-degree virtual casting closely approximated 
      corrosion casting studies. Tracer studies corroborated our findings. Tracer 
      decorated several larger vessels (50-100 mum) extending posteriorly from the 
      limbus in both raw and contrast-enhanced fluorescence images. Smaller 
      tracer-labeled vessels (30-40 mum) were seen branching between larger vessels and 
      exhibited a similar hexagonal network pattern. CONCLUSIONS: SD-OCT is capable of 
      detailed morphometric analysis of the conventional outflow pathway in vivo or ex 
      vivo with details comparable to corrosion casting techniques.
FAU - Francis, Andrew W
AU  - Francis AW
AD  - Department of Ophthalmology, Boston University School of Medicine, Boston, 
      Massachusetts, USA.
FAU - Kagemann, Larry
AU  - Kagemann L
FAU - Wollstein, Gadi
AU  - Wollstein G
FAU - Ishikawa, Hiroshi
AU  - Ishikawa H
FAU - Folz, Steven
AU  - Folz S
FAU - Overby, Darryl R
AU  - Overby DR
FAU - Sigal, Ian A
AU  - Sigal IA
FAU - Wang, Bo
AU  - Wang B
FAU - Schuman, Joel S
AU  - Schuman JS
LA  - eng
GR  - P30 EY008098/EY/NEI NIH HHS/United States
GR  - R01 EY013178/EY/NEI NIH HHS/United States
GR  - P30-EY08098/EY/NEI NIH HHS/United States
GR  - R01-EY13178/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20120807
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
SB  - IM
MH  - Anterior Chamber/blood supply
MH  - *Aqueous Humor
MH  - Cadaver
MH  - Corrosion Casting
MH  - Fluorescein Angiography
MH  - Humans
MH  - Imaging, Three-Dimensional
MH  - Microvessels/*anatomy & histology
MH  - Tomography, Optical Coherence/methods
MH  - Venules/anatomy & histology
PMC - PMC3727668
COIS- Disclosure: A.W. Francis, None; L. Kagemann, None; G. Wollstein, None; H. 
      Ishikawa, None; S. Folz, None; D.R. Overby, None; I.A. Sigal, None; B. Wang, 
      None; J.S. Schuman, P
EDAT- 2012/04/14 06:00
MHDA- 2012/10/25 06:00
PMCR- 2013/02/01
CRDT- 2012/04/14 06:00
PHST- 2012/04/14 06:00 [entrez]
PHST- 2012/04/14 06:00 [pubmed]
PHST- 2012/10/25 06:00 [medline]
PHST- 2013/02/01 00:00 [pmc-release]
AID - iovs.11-9229 [pii]
AID - 10.1167/iovs.11-9229 [doi]
PST - epublish
SO  - Invest Ophthalmol Vis Sci. 2012 Aug 7;53(9):5198-207. doi: 10.1167/iovs.11-9229.