PMID- 22500439
OWN - NLM
STAT- MEDLINE
DCOM- 20140702
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 29
IP  - 12
DP  - 2011 Dec
TI  - [Determination of aflatoxins in cashew by high performance liquid 
      chromatography-tandem mass spectrometry].
PG  - 1155-9
AB  - A method for the determination of four aflatoxins in cashew using high 
      performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was 
      developed. The sample was extracted with methanol-water (8: 2, v/v) solution, 
      followed by a cleanup procedure with Florisil column. The target compounds were 
      eluted using 5 mL acetone-water-formic acid (96: 3.5:0.5, v/v/v) solution. The 
      eluate was dried under N2, then dissolved in 1 mL methanol. Four aflatoxins were 
      separated in MG C18 column (100 mm x 3.0 mm, 3 microm) adopting a gradient 
      program within 15 min. A triple quadrupole mass spectrometry equipped with an 
      electrospray ionization source operated in the positive ion mode was used to 
      detect the aflatoxins. The good correlation coefficients (r2 > 0.997) of the four 
      aflatoxins were obtained within their respective linear ranges. The limits of 
      detection (S/N = 3) were between 0.009 microg/kg and 0.04 microg/kg, and the 
      limits of quantification (S/N = 10) were between 0.03 microg/kg and 0.12 
      microg/kg. The recoveries were in a range of 63.0% -78.5% with the relative 
      standard deviations (RSDs) varied from 2.8% to 9.1%. The validation results meet 
      the requirements of trace assay. Matrix effects were estimated and the signal 
      suppression/enhancement ranged from 88.8% to 99.4%. The results indicate that the 
      developed method is simple, fast, accurate, and can be applied for the 
      determination of fours aflatoxins in cashew.
FAU - Bi, Ruifeng
AU  - Bi R
AD  - College of Chemical Engineering, Qingdao University of Science and Technology, 
      Qingdao 266042, China. prefers@163.com
FAU - Fan, Zhixian
AU  - Fan Z
FAU - Fu, Meng
AU  - Fu M
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Aflatoxins)
SB  - IM
MH  - Aflatoxins/*analysis
MH  - Anacardium/*chemistry
MH  - *Chromatography, High Pressure Liquid
MH  - Food Contamination/*analysis
MH  - *Tandem Mass Spectrometry
EDAT- 2012/04/17 06:00
MHDA- 2014/07/06 06:00
CRDT- 2012/04/17 06:00
PHST- 2012/04/17 06:00 [entrez]
PHST- 2012/04/17 06:00 [pubmed]
PHST- 2014/07/06 06:00 [medline]
PST - ppublish
SO  - Se Pu. 2011 Dec;29(12):1155-9.