PMID- 22500448
OWN - NLM
STAT- MEDLINE
DCOM- 20140702
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 29
IP  - 12
DP  - 2011 Dec
TI  - [Rapid determination of propranolol enantiomers in rat plasma by 
      column-switching-high performance liquid chromatography].
PG  - 1205-9
AB  - A high performance liquid chromatographic (HPLC) method with column-switching was 
      developed and validated for rapid determination of two propranolol enantiomers in 
      rat plasma. The column of restricted-access media was used as a pre-treatment 
      column and a Chiralcel OD-RH was used as analytical column. The plasma samples 
      were injected directly into the pretreatment column to remove plasma protein and 
      endogenous constituents as well as to retent the propranolol enantiomers in the 
      column using the mobile phase of borate buffer (pH 8.5)-methanol (95:5, v/v) at 
      the flow rate of 1.0 mL/min. Then the propranolol enantiomers were transferred to 
      the Chiralcel OD-RH column using the mobile phase of isopropanol-ethanol-0.2 
      mmol/L borate buffer (pH 8.5) (30: 30: 40, v/v/v) at a flow rate of 0.8 mL/min by 
      column-switching technology. The column-switching time was 3.0 min, the used 
      wavelength was 293 nm and the column temperature was set at 25 degrees C. The 
      calibration curve showed excellent linear relationship (r = 0.999 5) in the 
      concentration range from 25 mg/L to 500 mg/L for propranolol enantiomers in 
      plasma. The intra-day and inter-day assay precisions and accuracies were well and 
      the relative standard deviations (RSDs) were less than 5%. The average recoveries 
      (n = 6) of the two enantiomers at 3 spiked levels were from 97.89% to 101.56%. 
      All the values of the method validation were within the generally accepted 
      criteria for biological sample analysis. The results show that the method is 
      convenient, quick, sensitive and accurate. The method was successfully applied in 
      the determination of propranolol enantiomers in rat blood pharmacokinetics study.
FAU - Wu, Xiaoyu
AU  - Wu X
AD  - Base for Clinical Pharmacology, Lanzhou General Hospital of Lanzhou Military 
      Region, Lanzhou 730050, China.
FAU - Wang, Rong
AU  - Wang R
FAU - Xie, Hua
AU  - Xie H
FAU - Wang, Jianfeng
AU  - Wang J
FAU - Jia, Zhengping
AU  - Jia Z
FAU - Zhang, Qiang
AU  - Zhang Q
FAU - Wang, Xianhua
AU  - Wang X
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 9Y8NXQ24VQ (Propranolol)
SB  - IM
MH  - Animals
MH  - Chromatography, High Pressure Liquid/instrumentation/*methods
MH  - Propranolol/*blood
MH  - Rats
MH  - Stereoisomerism
EDAT- 2012/04/17 06:00
MHDA- 2014/07/06 06:00
CRDT- 2012/04/17 06:00
PHST- 2012/04/17 06:00 [entrez]
PHST- 2012/04/17 06:00 [pubmed]
PHST- 2014/07/06 06:00 [medline]
PST - ppublish
SO  - Se Pu. 2011 Dec;29(12):1205-9.