PMID- 22500800
OWN - NLM
STAT- MEDLINE
DCOM- 20120608
LR  - 20220129
IS  - 1097-4172 (Electronic)
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 149
IP  - 2
DP  - 2012 Apr 13
TI  - Delineation of joint molecule resolution pathways in meiosis identifies a 
      crossover-specific resolvase.
PG  - 334-47
LID - 10.1016/j.cell.2012.03.023 [doi]
AB  - At the final step of homologous recombination, Holliday junction-containing joint 
      molecules (JMs) are resolved to form crossover or noncrossover products. The 
      enzymes responsible for JM resolution in vivo remain uncertain, but three 
      distinct endonucleases capable of resolving JMs in vitro have been identified: 
      Mus81-Mms4(EME1), Slx1-Slx4(BTBD12), and Yen1(GEN1). Using physical monitoring of 
      recombination during budding yeast meiosis, we show that all three endonucleases 
      are capable of promoting JM resolution in vivo. However, in mms4 slx4 yen1 triple 
      mutants, JM resolution and crossing over occur efficiently. Paradoxically, 
      crossing over in this background is strongly dependent on the Blooms helicase 
      ortholog Sgs1, a component of a well-characterized anticrossover activity. 
      Sgs1-dependent crossing over, but not JM resolution per se, also requires XPG 
      family nuclease Exo1 and the MutLgamma complex Mlh1-Mlh3. Thus, Sgs1, Exo1, and MutLgamma 
      together define a previously undescribed meiotic JM resolution pathway that 
      produces the majority of crossovers in budding yeast and, by inference, in 
      mammals.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Zakharyevich, Kseniya
AU  - Zakharyevich K
AD  - Department of Microbiology, University of California, Davis, One Shields Avenue, 
      Davis, CA 95616, USA.
FAU - Tang, Shangming
AU  - Tang S
FAU - Ma, Yunmei
AU  - Ma Y
FAU - Hunter, Neil
AU  - Hunter N
LA  - eng
GR  - HHMI_/Howard Hughes Medical Institute/United States
GR  - R01 GM074223/GM/NIGMS NIH HHS/United States
GR  - GM074223/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (DNA, Cruciform)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - EC 3.1.- (Endodeoxyribonucleases)
RN  - EC 3.1.21.- (Holliday Junction Resolvases)
RN  - EC 3.6.1.- (SGS1 protein, S cerevisiae)
RN  - EC 3.6.4.12 (RecQ Helicases)
SB  - IM
CIN - Cell. 2012 Apr 13;149(2):257-9. PMID: 22500794
MH  - *Crossing Over, Genetic
MH  - *DNA, Cruciform
MH  - Endodeoxyribonucleases/genetics/metabolism
MH  - Holliday Junction Resolvases/metabolism
MH  - *Meiosis
MH  - Mutation
MH  - RecQ Helicases/genetics/*metabolism
MH  - Saccharomyces cerevisiae/*cytology/*metabolism
MH  - Saccharomyces cerevisiae Proteins/genetics/*metabolism
PMC - PMC3377385
MID - NIHMS370774
EDAT- 2012/04/17 06:00
MHDA- 2012/06/09 06:00
PMCR- 2012/10/13
CRDT- 2012/04/17 06:00
PHST- 2011/10/10 00:00 [received]
PHST- 2012/01/31 00:00 [revised]
PHST- 2012/03/27 00:00 [accepted]
PHST- 2012/04/17 06:00 [entrez]
PHST- 2012/04/17 06:00 [pubmed]
PHST- 2012/06/09 06:00 [medline]
PHST- 2012/10/13 00:00 [pmc-release]
AID - S0092-8674(12)00399-6 [pii]
AID - 10.1016/j.cell.2012.03.023 [doi]
PST - ppublish
SO  - Cell. 2012 Apr 13;149(2):334-47. doi: 10.1016/j.cell.2012.03.023.