PMID- 22505415
OWN - NLM
STAT- MEDLINE
DCOM- 20120625
LR  - 20211021
IS  - 1744-3091 (Electronic)
IS  - 1744-3091 (Linking)
VI  - 68
IP  - Pt 4
DP  - 2012 Apr 1
TI  - Overexpression, crystallization and preliminary X-ray crystallographic analysis 
      of pyridoxal biosynthesis lyase PdxS from Pyrococcus horikoshii.
PG  - 440-2
LID - 10.1107/S1744309112005829 [doi]
AB  - Pyridoxal biosynthesis lyase (PdxS) is an important player in the biosynthesis of 
      pyridoxal 5'-phosphate (PLP), the biologically active form of vitamin B(6). PLP 
      is an important cofactor involved in the metabolic pathway of amine-containing 
      natural products such as amino acids and amino sugars. PdxS catalyzes the 
      condensation of ribulose 5-phosphate (Ru5P), glyceraldehyde 3-phosphate (G3P) and 
      ammonia, while glutamine amidotransferase (PdxT) catalyzes the production of 
      ammonia from glutamine. PdxS and PdxT form a complex, PLP synthase, and widely 
      exist in eubacteria, archaea, fungi and plants. To facilitate further structural 
      comparisons among PdxS proteins, the structural analysis of PdxS from Pyrococcus 
      horikoshii encoded by the Ph1355 gene was initiated. PdxS from P. horikoshii was 
      overexpressed in Escherichia coli and crystallized at 296 K using 
      2-methyl-2,4-pentanediol as a precipitant. Crystals of P. horikoshii PdxS 
      diffracted to 2.61 A resolution and belonged to the monoclinic space group P2(1), 
      with unit-cell parameters a = 59.30, b = 178.56, c = 109.23 A, beta = 102.97 degrees . The 
      asymmetric unit contained six monomers, with a corresponding V(M) of 2.54 A(3) 
      Da(-1) and a solvent content of 51.5% by volume.
CI  - (c) 2012 International Union of Crystallography. All rights reserved.
FAU - Yoon, Ji Young
AU  - Yoon JY
AD  - Department of Chemistry, College of Natural Sciences, Seoul National University, 
      Seoul 151-742, Republic of Korea.
FAU - Park, Chan Ryang
AU  - Park CR
FAU - Lee, Hyung Ho
AU  - Lee HH
FAU - Suh, Se Won
AU  - Suh SW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120327
PL  - England
TA  - Acta Crystallogr Sect F Struct Biol Cryst Commun
JT  - Acta crystallographica. Section F, Structural biology and crystallization 
      communications
JID - 101226117
RN  - EC 4.- (Lyases)
SB  - IM
MH  - Crystallization
MH  - Crystallography, X-Ray
MH  - Lyases/*chemistry
MH  - Pyrococcus horikoshii/*enzymology
PMC - PMC3325815
EDAT- 2012/04/17 06:00
MHDA- 2012/06/26 06:00
PMCR- 2014/04/01
CRDT- 2012/04/17 06:00
PHST- 2012/01/05 00:00 [received]
PHST- 2012/02/09 00:00 [accepted]
PHST- 2012/04/17 06:00 [entrez]
PHST- 2012/04/17 06:00 [pubmed]
PHST- 2012/06/26 06:00 [medline]
PHST- 2014/04/01 00:00 [pmc-release]
AID - S1744309112005829 [pii]
AID - fw5353 [pii]
AID - 10.1107/S1744309112005829 [doi]
PST - ppublish
SO  - Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Apr 1;68(Pt 4):440-2. doi: 
      10.1107/S1744309112005829. Epub 2012 Mar 27.